Human platelets loaded with trehalose survive freeze-drying
Human platelets loaded with trehalose survive freeze-drying
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DOI：
10.1006/cryo.2001.2306
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发表时间：
2001-03-01
期刊：
影响因子：
2.7
通讯作者：
Crowe, JH
中科院分区：
文献类型：
--
作者：
Wolkers, WF;Walker, NJ;Crowe, JH
根据联邦法规，人血小板在血库中储存5天，之后将其丢弃。这种短暂的寿命导致了血小板的长期短缺，这是一个在免疫抑制患者（如艾滋病患者）中特别严重的问题。我们在这里报告，血小板可以通过与海藻糖一起冷冻干燥来保存，海藻糖是一种在生物体中发现的高浓度糖，可以自然地在干燥中存活。我们认为这些发现将消除血小板的储存问题。海藻糖在37 ℃下被人血小板迅速吸收，负载效率为50%或更高。液相内吞作用在海藻糖的有效摄取中起重要作用，但也可能涉及其他机制。海藻糖负载的血小板被成功地冷冻干燥，具有良好的完整血小板的恢复。再水化从气相导致85%的存活率。这些血小板对激动剂凝血酶（1 U/ml）、胶原（2 μ g/ml）、ADP（20 μ M）和利托那韦（1.6 ms/ml）的反应几乎与新鲜的对照血小板相同。傅里叶变换红外光谱分析表明，海藻糖负载的血小板冻干后，预水化和再水化的膜和蛋白质成分是非常相似的新鲜血小板。(C)北京：科学出版社.
Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by Federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved by freeze-drying them with trehalose, a sugar Found at high concentrations in organisms that naturally survive drying. We suggest that these findings will obviate the storage problem with platelets. Trehalose is rapidly taken up by human platelets at 37 degreesC, with loading efficiencies of 50% or greater. Fluid-phase endocytosis plays an important role in this efficient uptake of trehalose, but other mechanisms may also be involved. Trehalose-loaded platelets were successfully freeze-dried, with excellent recovery of intact platelets. Rehydration From the vapor phase led to a survival rate of 85%. The response of these platelets to the agonists thrombin (1 U/ml), collagen (2 mug/ml), ADP (20 muM), and ristocetin (1.6 ms/ml) was almost identical to that of fresh, control platelets. Analysis by Fourier transform infrared spectroscopy demonstrated that the membrane and protein components of trehalose-loaded platelets after freeze-drying, prehydration, and rehydration were remarkably similar to those of fresh platelets. (C) 2001 Academic Press.