LONG-TERM COCAINE ADMINISTRATION IS NOT NEUROTOXIC TO CULTURED FETAL MESENCEPHALIC DOPAMINE NEURONS
LONG-TERM COCAINE ADMINISTRATION IS NOT NEUROTOXIC TO CULTURED FETAL MESENCEPHALIC DOPAMINE NEURONS
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DOI：
10.1016/0304-3940(93)90324-e
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发表时间：
1993-04-30
影响因子：
2.5
通讯作者：
CLODFELTER, JE
中科院分区：
文献类型：
--
作者：
BENNETT, BA;HYDE, CE;CLODFELTER, JE
精神兴奋剂可卡因和甲基苯丙胺通过与中脑边缘多巴胺系统相互作用产生欣快作用。与可卡因不同，甲基苯丙胺已被证明对多巴胺能和血清素能系统都具有神经毒性。我们之前已经确定，在我们的中脑组织培养模型中，接触甲基苯丙胺 6 天会导致酪氨酸羟化酶免疫阳性细胞的神经元损伤。在相同的暴露时间内，可卡因既不会损害神经元功能，也不会改变多巴胺细胞的存活。为了测试较长时间接触可卡因是否会改变多巴胺功能，我们每天一次向培养物中添加可卡因（100 μM），持续 8 或 11 天，并在最后一次给药后 24 小时检查多巴胺摄取、细胞存活和形态的变化。可卡因在中脑培养物中没有产生任何神经毒性迹象。
The psychostimulants cocaine and methamphetamine produce their euphoric effects through an interaction with the mesolimbic dopamine system. Methamphetamine, unlike cocaine, has been shown to be neurotoxic to both dopaminergic and serotonergic systems. We have previously determined that a 6 day exposure to methamphetamine causes neuronal damage to tyrosine hydroxylase-immunopositive cells in our tissue culture model of the mesencephalon. Over the same exposure period, cocaine neither impaired neuronal function nor altered dopamine cell survival. To test whether a longer exposure period to cocaine would alter dopamine function, we added cocaine (100 muM) to the cultures once daily for either 8 or 11 days and examined changes in dopamine uptake, cell survival and morphology 24 h after the last administration. Cocaine did not produce any signs of neurotoxicity in the mesencephalic cultures.