Retrovirus-mediated gene transfer to retinal explants
Retrovirus-mediated gene transfer to retinal explants
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DOI：
10.1016/s1046-2023(02)00257-8
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发表时间：
2002-12-01
期刊：
影响因子：
4.8
通讯作者：
Kageyama, R
中科院分区：
文献类型：
--
作者：
Hatakeyama, J;Kageyama, R
可以从小鼠胚胎中分离出神经视网膜，并在培养中保持2-3周。在这种视网膜外植体培养中，前体细胞分化为神经元和神经胶质细胞，形成三层细胞层，很好地模拟了正常发育。该外植体培养体系适用于逆转录病毒介导的基因转移等遗传操作。逆转录病毒载体可以有效地将基因转移到视网膜前体细胞，并且病毒基因组的副本被精确地传播到受感染细胞的后代。因此，这是一种稳定改变分裂细胞表型的好方法。已有研究表明，携带转录因子基因的逆转录病毒可有效改变受感染细胞的命运。逆转录病毒载体的双顺反子表达有助于测试多种转录因子的不同组合的效果。用这种方法，视网膜细胞类型规范的转录密码现在正在被阐明。因此，逆转录病毒介导的视网膜外植体培养系统的基因转移为分析神经发育的分子机制提供了一个强大而独特的工具。(C)2002年埃尔塞维尔科学公司(美国)。版权所有。
Neural retina can be isolated from mouse embryos and maintained in culture for 2-3 weeks. In such retinal explant cultures, precursor cells differentiate into neurons and glial cells and form three cellular layers, mimicking well the normal development. This explant culture system is suitable for genetic manipulation, such as retrovirus-mediated gene transfer. Retroviral vectors can efficiently transfer genes into retinal precursors, and the copy of the viral genome is precisely transmitted to the progeny of infected cells. Thus, this is an excellent method to change stably the phenotypes of dividing cells. It has been shown that retroviruses carrying transcription factor genes efficiently change the fates of infected cells. Bicistronic expression by retroviral vectors is useful to test the effects of various combinations of many transcription factors. With this method, the transcriptional codes for retinal cell type specification are now being elucidated. Thus, retrovirus-mediated gene transfer to the retinal explant culture system offers a powerful and unique tool to analyze the molecular mechanism of neural development. (C) 2002 Elsevier Science (USA). All rights reserved.