Aphelenchoides besseyi Ab-FAR-1 Interacts with Arabidopsis thaliana AtADF3 to Interfere with Actin Cytoskeleton, and Promotes Nematode Parasitism and Pathogenicity.
Aphelenchoides besseyi Ab-FAR-1 Interacts with Arabidopsis thaliana AtADF3 to Interfere with Actin Cytoskeleton, and Promotes Nematode Parasitism and Pathogenicity.
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贝氏滑刃线虫 Ab-FAR-1 与拟南芥 AtADF3 相互作用干扰肌动蛋白细胞骨架，促进线虫寄生和致病性
DOI：
10.3390/ijms232012280
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发表时间：
2022-10-14
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
脂肪酸和视黄醇结合蛋白(FAR)是在线虫中发现的独特蛋白质，被认为是控制这些寄生虫的潜在靶标。然而，它们在线虫寄生、致病和与寄主相互作用中的作用尚不清楚。在这项研究中，我们研究了水稻白尖线虫(RWTN)和Aphelenchoidesseyi蛋白的特殊作用，以阐明线虫的寄生和致病过程。结果表明，白僵菌侵染植物后，AbFAR-1的表达水平显著上调。免疫荧光和亚细胞定位表明，AbFar-1主要通过线虫的体壁分泌到植物组织中，可能作用于植物细胞的胞核和细胞质。RWTN在高表达AbFar-1的拟南芥中的致病力增强，而在AbFar-1RNAi A.thaliana中的致病力受到抑制。酵母双杂交、Co-IP、BIFC和线虫接种实验表明，AbFar-1能与拟南芥肌动蛋白解聚因子蛋白AtADF3相互作用，并且该突变株对线虫更敏感。体外肌动蛋白细丝解聚实验表明，AbFAR-1可以抑制AtADF3介导的肌动蛋白细丝解聚，同时也影响了过表达AbFAR-1的拟南芥细胞肌动蛋白细丝的周转过程。此外，在过量表达Ab-Far-1和Adf3的突变体中，flg22介导的宿主防御反应受到抑制。因此，本研究证实RWTN可以通过分泌Ab-Far-1来影响AtADF3介导的肌动蛋白细丝重构的周转，从而抑制植物PAMP触发的免疫(PTI)，促进线虫的寄生和致病。
Fatty acid and retinol binding proteins (FAR) are unique proteins found in nematodes and are considered potential targets for controlling these parasites. However, their functions in nematode parasitism and pathogenicity and interaction with hosts are still unclear. In this study, we investigated the specific roles of rice white tip nematodes (RWTNs), Aphelenchoides besseyi, and a protein, Ab-FAR-1, to elucidate the parasitic and pathogenic processes of nematodes. The results showed that the expression level of Ab-far-1 was significantly up-regulated after A. besseyi infection of the plant. The immunofluorescence and subcellular localisation showed that Ab-FAR-1 was secreted into plant tissues mainly through the body wall of nematodes and might act in the nucleus and cytoplasm of plant cells. The pathogenicity of RWTNs was enhanced in Arabidopsis thaliana overexpressing Ab-FAR-1 and inhibited in Ab-far-1 RNAi A. thaliana. Yeast two-hybrid, Co-IP, BiFC, and nematode inoculation experiments showed that Ab-FAR-1 could interact with the A. thaliana actin-depolymerizing factor protein AtADF3, and the A. thaliana adf3 mutant was more susceptible to nematodes. An in vitro actin filament depolymerisation assay demonstrated that Ab-FAR-1 could inhibit AtADF3-mediated depolymerisation of actin filaments, and the turnover process of cellular actin filaments was also affected in A. thaliana overexpressing Ab-FAR-1. In addition, flg22-mediated host defence responses were suppressed in A. thaliana overexpressing Ab-FAR-1 and adf3 mutants. Therefore, this study confirmed that RWTNs can affect the turnover of actin filament remodelling mediated by AtADF3 through Ab-FAR-1 secretion and thus inhibit plant PAMP-triggered immunity (PTI), promoting the parasitism and pathogenicity of nematodes.
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影响因子：
4
作者：
Fairfax KC;Vermeire JJ;Harrison LM;Bungiro RD;Grant W;Husain SZ;Cappello M
通讯作者：
Cappello M
影响因子：
4.6
作者：
Wang DW;Peng XF;Xie H;Xu CL;Cheng DQ;Li JY;Wu WJ;Wang K
通讯作者：
Wang K
影响因子：
5.6
作者：
Barletta, G. P.;Franchini, G.;Fernandez-Alberti, S.
通讯作者：
Fernandez-Alberti, S.
影响因子：
1.5
作者：
Basavaraju, S;Zhan, B;Hotez, PJ
通讯作者：
Hotez, PJ
影响因子：
16
作者：
Andrianantoandro, Ernesto;Pollard, Thomas D.
通讯作者：
Pollard, Thomas D.