Effect of age on surface molecules and cytokine expression in human dendritic cells
Effect of age on surface molecules and cytokine expression in human dendritic cells
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DOI：
10.1016/j.cellimm.2011.04.010
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发表时间：
2011-01-01
影响因子：
4.3
通讯作者：
Bossu, Paola
中科院分区：
文献类型：
--
作者：
Ciaramella, Antonio;Spalletta, Gianfranco;Bossu, Paola
树突状细胞（DC）在调节先天性和获得性免疫方面发挥着核心作用，但其可能与年龄相关的功能修饰仍不清楚。在这里，我们分析了年龄对 LPS 处理的单核细胞来源的 DC (MDDC) 的影响。在一组健康捐献者中观察到年龄与 ICAM-1、CD25 和 IL-10 的细胞表达呈负相关。与年轻个体相比，老年亚组细胞中相同分子的表达显着降低证实了这一点。相反，在所有受试者中，年龄与 IL-6 和 IL-18 的细胞表达之间存在正相关性，并且老年受试者细胞中两种促炎细胞因子的显着增加进一步证明了这一点。我们的数据表明，衰老会损害 ICAM-1 和 CD25 的表达，并扭曲细胞因子（包括 IL-18）的产生，而 IL-18 会共同形成促炎环境。总而言之，目前的结果指出了其他分子的作用可能与人类树突状细胞的免疫衰老相关，证实这些细胞在衰老过程中经历功能变化。 (C) 2011 Elsevier Inc. 保留所有权利。
Dendritic cells (DCs) are central in regulating both innate and acquired immunity, but their possible age-related functional modifications are still unclear. Here we have analyzed the effect of age on LPS-treated monocyte-derived DCs (MDDCs). A negative correlation between age and cell expression of ICAM-1, CD25 and IL-10 was observed in a group of healthy donors. This has been confirmed by a significantly reduced expression of the same molecules in cells of subgrouped elderly versus younger individuals. On the contrary, a positive correlation between age and cell expression of IL-6 and IL-18 has been reported in all the subjects and further supported by a significant increase of the two pro-inflammatory cytokines in cells of elderly versus young subjects.Our data indicate that aging can impair the expression of ICAM-1 and CD25 and skew the production of cytokines, including IL-18, which concur to make a pro-inflammatory milieu. Altogether, the present results point to additional molecules whose role might be relevant in immunosenescence of human DCs, confirming that these cells undergo functional changes during aging. (C) 2011 Elsevier Inc. All rights reserved.