Decreased plasma cartilage acidic protein 1 in COVID-19.
Decreased plasma cartilage acidic protein 1 in COVID-19.
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DOI：
10.14814/phy2.15814
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发表时间：
2023-09
影响因子：
2.5
通讯作者：
中科院分区：
文献类型：
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作者：
酸性软骨蛋白 - 1（CRTAC1）由多种细胞类型产生，包括被新冠病毒（SARS - CoV2）靶向的2型肺泡上皮（T2AE）细胞。基于蛋白质组学研究可知，重症新冠肺炎患者的血浆CRTAC1水平较低。通过酶联免疫吸附测定（ELISA），我们发现重症监护病房（ICU）中接受新冠肺炎治疗的患者，其血浆CRTAC1浓度几乎都低于健康对照组。CRTAC1下降的幅度可将新冠肺炎与其他导致急性呼吸衰竭的原因区分开来，并且与已确定的新冠肺炎严重程度指标相关。在一些新冠肺炎住院一年后的患者、病情较轻的新冠肺炎后出现的长新冠患者或慢性阻塞性肺疾病患者中，发现CRTAC1浓度低于对照组。重症新冠肺炎患者中CRTAC1的下降与CFP（备解素）的下降相关（r = 0.37，p = 0.0001），CFP与CRTAC1相互作用。因此，与重症新冠肺炎相关的CRTAC1下降可能是由于T2AE细胞产生减少或与CFP共同耗竭所致。要确定长新冠患者亚群中CRTAC1浓度下降的意义及其背后的原因，需要分析大量患者中先前存在的肺部疾病的作用、既往急性新冠肺炎的影响、年龄以及其他混杂变量。
一种双位点ELISA检测了急性和长新冠患者中CRTAC1浓度相对于正常范围（阴影部分）的偏差。CRTAC1的下降与CFP的下降相关，在相互作用ELISA中CRTAC1与CFP结合。
Cartilage acidic protein‐1 (CRTAC1) is produced by several cell types, including Type 2 alveolar epithelial (T2AE) cells that are targeted by SARS‐CoV2. Plasma CRTAC1 is known based on proteomic surveys to be low in patients with severe COVID‐19. Using an ELISA, we found that patients treated for COVID‐19 in an ICU almost uniformly had plasma concentrations of CRTAC1 below those of healthy controls. Magnitude of decrease in CRTAC1 distinguished COVID‐19 from other causes of acute respiratory decompensation and correlated with established metrics of COVID‐19 severity. CRTAC1 concentrations below those of controls were found in some patients a year after hospitalization with COVID‐19, long COVID after less severe COVID‐19, or chronic obstructive pulmonary disease. Decreases in CRTAC1 in severe COVID‐19 correlated (r = 0.37, p = 0.0001) with decreases in CFP (properdin), which interacts with CRTAC1. Thus, decreases of CRTAC1 associated with severe COVID‐19 may result from loss of production by T2AE cells or co‐depletion with CFP. Determination of significance of and reasons behind decreased CRTAC1 concentration in a subset of patients with long COVID will require analysis of roles of preexisting lung disease, impact of prior acute COVID‐19, age, and other confounding variables in a larger number of patients. A double‐site ELISA measured deviations in the concentration of CRTAC1 from the normal range (shaded) in patients with acute and long COVID‐19. Decreases in CRTAC1 correlated with decreases in CFP, to which CRTAC1 bound in an interaction ELISA.
在致命的Covid-19中，与年龄相关风险和肺泡损伤的免疫和上皮决定因素。
影响因子：
8
作者：
Chait, Michael;Yilmaz, Mine M.;Shakil, Shanila;Ku, Amy W.;Dogra, Pranay;Connors, Thomas J.;Szabo, Peter A.;Gray, Joshua I.;Wells, Steven B.;Kubota, Masaru;Matsumoto, Rei;Poon, Maya M. L.;Snyder, Mark E.;Baldwin, Matthew R.;Sims, Peter A.;Saqi, Anjali;Farber, Donna L.;Weisberg, Stuart P.
通讯作者：
Weisberg, Stuart P.