Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy
Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy
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DOI：
10.1016/j.colsurfb.2011.05.013
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发表时间：
2011-10-01
影响因子：
5.8
通讯作者：
Glebel, Bernd
中科院分区：
文献类型：
--
作者：
Sokolova, Viktoriya;Ludwig, Anna-Kristin;Glebel, Bernd
通过扫描电子显微镜（SEM）、动态光散射（DLS）和纳米颗粒跟踪分析（NTA）表征来自三种不同细胞类型（HEK 293T、ECFC、MSC）的外来体。三种细胞类型的直径约为110 nm。在-20 ℃、4 ℃和37 ℃下储存期间检查外泌体的稳定性。外泌体的大小在4 ℃和37 ℃时减小，表明结构变化或降解。多次冷冻至-20 ℃和解冻并不影响外泌体的大小。多次超离心也没有改变外泌体的大小。(C)2011爱思唯尔有限公司版权所有。
Exosomes from three different cell types (HEK 293T, ECFC, MSC) were characterised by scanning electron microscopy (SEM), dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). The diameter was around 110 nm for the three cell types. The stability of exosomes was examined during storage at -20 degrees C, 4 degrees C, and 37 degrees C. The size of the exosomes decreased at 4 degrees C and 37 degrees C, indicating a structural change or degradation. Multiple freezing to -20 degrees C and thawing did not affect the exosome size. Multiple ultracentrifugation also did not change the exosome size. (C) 2011 Elsevier B.V. All rights reserved.