Maternal protein restriction during pregnancy and lactation affects the development of muscle fibers and neuromuscular junctions in rats
Maternal protein restriction during pregnancy and lactation affects the development of muscle fibers and neuromuscular junctions in rats
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DOI：
10.1002/mus.25187
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发表时间：
2017-01-01
期刊：
影响因子：
3.4
通讯作者：
Torrejais, Marcia Miranda
中科院分区：
文献类型：
--
作者：
Confortim, Heloisa Deola;Jeronimo, Leslie Cazetta;Torrejais, Marcia Miranda
简介：均衡的母亲饮食是胎儿正常发育的决定性因素。本研究旨在探讨妊娠期和哺乳期母体蛋白限制对21日龄子代大鼠肌纤维和神经肌肉接头（NMJ）形态的影响。方法：将Wistar大鼠分为对照组（CG）和限制性组（RG），前者为正常蛋白饲粮母鼠的后代（17%），后者为低蛋白饲粮母鼠的后代（6%）。哺乳一段时间后，对动物实施安乐死，并从幼崽身上获得比目鱼肌进行分析。结果：RG比目鱼肌纤维数量增加133%，核数量增加79%。此外，限制组的NMJs数量低于CG组。结论：母体蛋白限制改变了神经肌肉系统的正常发育。中国生物医学工程学报（英文版），2017
Introduction: A balanced maternal diet is a determining factor in normal fetal development. The objective of this study was to evaluate the effects of maternal protein restriction during pregnancy and lactation on muscle fiber and neuromuscular junction (NMJ) morphology of rat offspring at 21 days of age. Methods: Wistar rats were divided into a control group (CG), offspring of mothers fed a normal protein diet (17%), and a restricted group (RG), offspring of mothers fed a low-protein diet (6%). After a period of lactation, the animals were euthanized, and soleus muscles were obtained from pups for analysis. Results: The soleus muscles of the RG exhibited an increase of 133% in the number of fibers and of 79% in the amount of nuclei. Moreover, the number of NMJs was lower in the restricted group than in the CG. Conclusions: Maternal protein restriction alters the normal development of the neuromuscular system. Muscle Nerve55: 109-115, 2017