Comparative study on low resolution structures of apoferritin via SANS and SAXS
Comparative study on low resolution structures of apoferritin via SANS and SAXS
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DOI：
10.1088/1742-6596/351/1/012009
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发表时间：
2012-03
期刊：
影响因子：
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通讯作者：
A. I. Kuklin;T. Murugova;O. Ivankov;A. Rogachev;D. Soloviov;Yu. S. Kovalev;A V Ishchenko;A. Zhigunov;T. S. Kurkin;V. Gordeliy
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文献类型：
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作者：
A. I. Kuklin;T. Murugova;O. Ivankov;A. Rogachev;D. Soloviov;Yu. S. Kovalev;A V Ishchenko;A. Zhigunov;T. S. Kurkin;V. Gordeliy
介绍了载铁蛋白的小角散射研究结果。计算了包括间接傅里叶变换法确定的尺寸和形状。得到了小角中子（SANS）和x射线小角散射（SAXS）的对距分布函数。结果表明，SANS和SAXS方法得到的载铁蛋白形状相似（带孔的球形壳）。同时，对SAXS曲线和SANS曲线的实验数据进行拟合，得出分子的大小和体积略有不同。讨论了产生这些差异的原因。
The results of small angle scattering investigation of protein apoferritin are presented. The sizes and shapes, including those determined by indirect Fourier transform method, are calculated. The pair-distance distribution function for both small angle neutron (SANS) and X-ray small angle scattering (SAXS) are obtained. It is shown that SANS and SAXS methods give similar shape (spherical shell with holes) of apoferritin. At the same time, fits of experimental data for SAXS and SANS curves give a little different sizes and volumes of the molecule. The reasons for these differences are discussed.