Polyurethane with tethered copper(II)-cyclen complex:: Preparation, characterization and catalytic generation of nitric oxide from S-nitrosothiols
Polyurethane with tethered copper(II)-cyclen complex:: Preparation, characterization and catalytic generation of nitric oxide from S-nitrosothiols
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DOI：
10.1016/j.biomaterials.2008.02.004
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发表时间：
2008-06-01
期刊：
影响因子：
14
通讯作者：
Meyerhoff, Mark E.
中科院分区：
文献类型：
--
作者：
Hwang, Sangyeul;Meyerhoff, Mark E.
介绍了一种商业生物医用级聚氨酯(Tecophilic(R)，SP-93A-100)材料的制备和表征，该材料具有共价连接的铜(II)-环烯基团作为产生一氧化氮(NO)的聚合物。化学发光NO测量表明，在生理pH条件下，在硫醇还原剂(RSHS；谷胱甘肽和半胱氨酸)的存在下，合成的聚合物能将内源的S-亚硝硫醇(如S-亚硝基谷胱甘肽和S-亚硝半胱氨酸)分解为NO。由于这种RSNO和RSH物种已经存在于血液中，所提出的聚合物在与新鲜血液接触时能够自发产生NO。这是通过利用聚合物作为电流型一氧化氮传感器远端的外层涂层来创建一种对血液中的RSNO物种产生反应的设备来实现的。这种聚合物兼具商业生物医用级聚氨酯的优点和与血液接触时产生生物活性NO的能力，因此可以作为一种有用的涂层来改善各种医疗设备的血液相容性。(C)2008爱思唯尔有限公司。保留所有权利。
The preparation and characterization of a commercial biomedical-grade polyurethane (Tecophilic (R), SP-93A-100) material possessing covalently linked copper(II)-cyclen moieties as a nitric oxide (NO) generating polymer are described. Chemiluminescence NO measurements demonstrate that the prepared polymer can decompose endogenous S-nitrosothiols (RSNOs) such as S-nitrosoglutathione and S-nitrosocysteine to NO in the presence of thiol reducing agents (RSHs; e.g., glutathione and cysteine) at physiological pH. Since such RSNO and RSH species already exist in blood, the proposed polymer is capable of spontaneously generating NO when in contact with fresh blood. This is demonstrated by utilizing the polymer as an outer coating at the distal end of an amperometric NO sensor to create a device that generates response toward the RSNO species in the blood. This polymer possesses the combined benefits of a commercial biomedical-grade polyurethane with the ability to generate biologically active NO when in contact with blood, and thus may serve as a useful coating to improve the hemocompatibility of various medical devices. (c) 2008 Elsevier Ltd. All rights reserved.