Engineering precision RNA molecular switches
Engineering precision RNA molecular switches
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DOI：
10.1073/pnas.96.7.3584
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发表时间：
1999-03-30
影响因子：
11.1
通讯作者：
Breaker, RR
中科院分区：
文献类型：
--
作者：
Soukup, GA;Breaker, RR
由RNA组成的配体特异性分子开关通过结构桥将预先存在的催化结构域和受体结构域偶联而产生。配体与受体的结合引发了桥内的构象变化，这种结构重组决定了相邻核酶的活性。这些三部分构建体的模块化性质使得快速构建仅在其相应配体存在下触发的精确RNA分子开关成为可能。通过使用类似的酶工程策略，可以使新的RNA开关作为设计分子传感器或作为一类新的遗传控制元件。
Ligand-specific molecular switches composed of RNA were created by coupling preexisting catalytic and receptor domains via structural bridges. Binding of ligand to the receptor triggers a conformational change within the bridge, and this structural reorganization dictates the activity of the adjoining ribozyme. The modular nature of these tripartite constructs makes possible the rapid construction of precision RNA molecular switches that trigger only in the presence of their corresponding ligand. By using similar enzyme engineering strategies, new RNA switches can be made to operate as designer molecular sensors or as a new class of genetic control elements.