Genome-wide association study identifies candidate genes related to oleic acid content in soybean seeds
Genome-wide association study identifies candidate genes related to oleic acid content in soybean seeds
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DOI：
10.1186/s12889-020-09277-1
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发表时间：
2020-08-28
影响因子：
5.3
通讯作者：
Wang, Piwu
中科院分区：
文献类型：
--
作者：
Liu, Xiaoyi;Qin, Di;Wang, Piwu
大豆油是五种脂肪酸（棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸）的复杂混合物。具有高油酸含量的大豆油是理想的，因为这种单不饱和脂肪酸改善了油的氧化稳定性。以东北地区260份大豆种质为材料，研究了大豆种子油酸的遗传结构。对260份种质资源进行了全基因组关联研究（GWAS）。结果表型鉴定结果表明，油酸含量在8.2%~ 35.0%之间。共获得2，311，337个单核苷酸多态性（SNP）标记。GWAS分析表明，与油酸含量相关的基因较多，贡献率约为7%。在2年的GWAS中检测到位于第11染色体上的候选基因Glyma.11G229600.1和位于第4染色体上的候选基因Glyma.04G102900.1。候选基因Glyma.11G229600.1与油酸含量呈正相关，相关系数为0.980; Glyma.04G102900.1与油酸含量呈负相关，相关系数为-0.964。结论2018年和2019年两次分析均检测到4号染色体上的Glyma.04G102900.1和11号染色体上的Glyma.11G229600.1，Glyma.04G102900.1和Glyma.11G229600.1是大豆种子油酸相关的新的关键候选基因。这些结果将有助于高油酸大豆育种。
Background Soybean oil is a complex mixture of five fatty acids (palmitic, stearic, oleic, linoleic, and linolenic). Soybean oil with a high oleic acid content is desirable because this monounsaturated fatty acid improves the oxidative stability of the oil. To investigate the genetic architecture of oleic acid in soybean seeds, 260 soybean germplasms from Northeast China were collected as natural populations. A genome-wide association study (GWAS) was conducted on a panel of 260 germplasm resources. Results Phenotypic identification results showed that the oleic acid content varied from 8.2 to 35.0%. A total of 2,311,337 single-nucleotide polymorphism (SNP) markers were obtained. GWAS analysis showed that there were many genes related to oleic acid content with a contribution rate of 7%. The candidate genesGlyma.11G229600.1on chromosome 11 andGlyma.04G102900.1on chromosome 4 were detected in a 2-year-long GWAS. The candidate geneGlyma.11G229600.1showed a positive correlation with the oleic acid content, and the correlation coefficient was 0.980, whileGlyma.04G102900.1showed a negative correlation, with a coefficient of - 0.964. Conclusions Glyma.04G102900.1on chromosome 4 andGlyma.11G229600.1on chromosome 11 were detected in both analyses (2018 and 2019).Glyma.04G102900.1andGlyma.11G229600.1are new key candidate genes related to oleic acid in soybean seeds. These results will be useful for high-oleic soybean breeding.