Expression of tropomyosin alpha 4 chain is increased in esophageal squamous cell carcinoma as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry
Expression of tropomyosin alpha 4 chain is increased in esophageal squamous cell carcinoma as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry
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DOI：
10.1002/prca.200600609
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发表时间：
2007-02-01
影响因子：
2
通讯作者：
Nakamura, Kazuyuki
中科院分区：
文献类型：
--
作者：
Harada, Toshio;Kuramitsu, Yasuhiro;Nakamura, Kazuyuki
目的：鉴定食管癌相关蛋白。为探讨食管鳞癌的发生、发展过程，我们对16例食管鳞状细胞癌（ESCCs）及其癌旁组织进行了双向电泳和MS/MS分析。通过PMF和LC-MS/MS确定斑点的同一性，并通过免疫印迹法进行确认。表达上调的蛋白质为原肌球蛋白α 4链、转凝胶蛋白和丙酮酸激酶。下调的蛋白质是血清白蛋白前体、膜联蛋白A1亚型、原肌球蛋白β链、14-3-3蛋白σ和血清转铁蛋白前体亚型。在所有16例病例中，通过免疫印迹证实了原肌球蛋白α 4链的上调。原肌球蛋白α 4链在食管鳞癌细胞和邻近的成纤维细胞中的定位通过免疫组织化学证实。
To identify proteins associated with esophageal. carcinogenesis, we performed protein profiling of 16 esophageal squamous cell carcinomas (ESCCs) and paired noncancerous tissues by 2-DE and MS/MS. In cancerous tissues, three spots showed significant up-regulation in the amount of protein, while eight spots were significantly down-regulated. The identities of the spots were determined by PMF with LC-MS/MS and were confirmed by immunoblotting. The up-regulated proteins were tropomyosin alpha 4 chain, transgelin, and pyruvate kinase. The down-regulated proteins were serum albumin precursor, isoforms of annexin A1, tropomyosin beta chain, 14-3-3 protein sigma, and isoforms of serotransferrin precursor. In all 16 cases, up-regulation of the tropomyosin alpha 4 chain was confirmed by immunoblotting. Localization of the tropomyosin alpha 4 chain in ESCC cells and adjacent fibroblasts was confirmed by immunohistochemistry.