Evaluation of interaction between Brachypodium distachyon roots and Fusarium species
Evaluation of interaction between Brachypodium distachyon roots and Fusarium species
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DOI：
10.1007/s10658-020-02159-7
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发表时间：
2020-11-21
影响因子：
1.8
通讯作者：
Stenglein, Sebastian A.
中科院分区：
文献类型：
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作者：
Dinolfo, Maria, I;Martinez, Mauro;Stenglein, Sebastian A.
镰刀菌头枯病是世界范围内最重要的谷物病害。不同的花/谷物致病镰刀菌物种之间的相互作用的研究已经进行，使用的主机和组织，从该真菌被分离。本研究旨在评估二穗短柄草（Brachypodium distachyon）基因型根系对镰刀菌（Fusarium spp.）以提供关于宿主抗性和/或真菌侵袭性的差异的信息。共筛选出4种镰刀菌的10个菌株：F.禾谷镰孢F. pseudograminearum，F. cerealis和F. Poae。共7种B基因型。使用的基因型为：阿富汗、伊朗、以色列、巴基斯坦、南非、乌拉圭和Bd基因型21。接种根，并在接种后2、4、6和8天测量坏死根长度。结果表明，F. graminearum和F. pseudograminearum的AUDPC值最高，其次是F.禾谷类植物，显示AUDPC的中间值。F. Poae是攻击性最低的物种。关于短柄草属，受影响最小的基因型是Bd 21和巴基斯坦，而其余的基因型表现出易感性的差异。据我们所知，这是第一个报告表明F。pseudograminearum，F. cerealis和F.早熟禾具有短柄草属的根。此外，报道了短柄草属基因型和镰刀菌属物种侵略性之间的易感性的变异。我们得出结论，B.二穗草根提供了一个有效的组织系统模型，以评估与镰刀菌的相互作用。
Fusarium Head Blight is the most important disease of cereals worldwide. Studies of interactions between different flower/grain pathogenic Fusarium species have been carried out, using the host and tissue from which the fungus was isolated. The aim of the present study was to assess the root responses of Brachypodium distachyon genotypes to Fusarium spp. to provide information about differences in host resistance and/or fungal aggressiveness. A total of 10 isolates of four Fusarium species were selected: F. graminearum, F. pseudograminearum, F. cerealis, and F. poae. A total of seven genotypes of B. distachyon were used: Afghanistan, Iran, Israel, Pakistan, South Africa, Uruguay and Bd genotype 21. Roots were inoculated and the necrosis root length was measured at 2, 4, 6, and 8 days post-inoculation. The results showed that F. graminearum and F. pseudograminearum were the species with the highest AUDPC values followed by F. cerealis that showed an intermediate value of AUDPC. F. poae was the least aggressive species. Regarding Brachypodium, the least affected genotype was Bd 21 and Pakistan, while the remaining genotypes showed differences in susceptibility. To our knowledge, this is the first report showing interaction between F. pseudograminearum, F. cerealis and F. poae with Brachypodium roots. Moreover, variability in susceptibility among Brachypodium genotypes and in Fusarium species aggressiveness was reported. We concluded that B. distachyon roots offer an efficient tissue system model to evaluate interaction with Fusarium spp.