A microfabricated platform to form three-dimensional toroidal multicellular aggregate
A microfabricated platform to form three-dimensional toroidal multicellular aggregate
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DOI：
10.1007/s10544-012-9713-0
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发表时间：
2012-12-01
影响因子：
2.8
通讯作者：
Arai, Fumihito
中科院分区：
文献类型：
--
作者：
Masuda, Taisuke;Takei, Natsuki;Arai, Fumihito
允许细胞自组装成三维（3D）球体微组织的技术提供了强大的体外模型，这些模型在干细胞研究、组织工程和癌症生物学等领域越来越受欢迎。组织正常发育和功能的3D环境的适当模拟对于可用于形成复杂组织的体外模型的工程设计至关重要。我们已经开发出一种独特的多细胞聚集体形成平台，利用无掩模灰度光刻。使用该平台形成的细胞聚集体具有环形几何形状，并且包括促进氧气和生长因子的供应以及废物的排出的微腔。因此，该平台能够一次快速产生数百个多细胞聚集体，并通过复杂的设计调节聚集体的直径。这些环形多细胞聚集体可以作为长期培养物生长。此外，微腔可用作连续通道，并用于将血管系统或神经系统插入组装的组织中。这些平台特性突出了其在各种应用中的潜力，例如作为生物致动器，作为微机械部件或用于药物筛选和组织工程。
Techniques that allow cells to self-assemble into three-dimensional (3D) spheroid microtissues provide powerful in vitro models that are becoming increasingly popular in fields such as stem cell research, tissue engineering, and cancer biology. Appropriate simulation of the 3D environment in which tissues normally develop and function is crucial for the engineering of in vitro models that can be used for the formation of complex tissues. We have developed a unique multicellular aggregate formation platform that utilizes a maskless gray-scale photolithography. The cellular aggregate formed using this platform has a toroidal-like geometry and includes a micro lumen that facilitates the supply of oxygen and growth factors and the expulsion of waste products. As a result, this platform was capable of rapidly producing hundreds of multicellular aggregates at a time, and of regulating the diameter of aggregates with complex design. These toroidal multicellular aggregates can grow as long-term culture. In addition, the micro lumen can be used as a continuous channel and for the insertion of a vascular system or a nerve system into the assembled tissue. These platform characteristics highlight its potential to be used in a wide variety of applications, e.g. as a bioactuator, as a micro-machine component or in drug screening and tissue engineering.