Exploring the interaction between polystyrene nanoplastics and Allium cepa during germination: Internalization in root cells, induction of toxicity and oxidative stress
Exploring the interaction between polystyrene nanoplastics and Allium cepa during germination: Internalization in root cells, induction of toxicity and oxidative stress
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DOI：
10.1016/j.plaphy.2020.02.014
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发表时间：
2020-04-01
影响因子：
6.5
通讯作者：
Castiglione, Monica Ruffini
中科院分区：
文献类型：
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作者：
Giorgetti, Lucia;Spano, Carmelina;Castiglione, Monica Ruffini
为了研究纳米塑料（纳米PS）对植物的作用机制，洋葱种子发芽72小时，在聚苯乙烯纳米PS（50 nm大小，浓度为0.01，0.1和1克L-1）的存在下，随后，根进行了分析，通过多方面的方法。任何浓度的纳米PS对种子发芽率均无影响，而0.1和1 g L-1纳米PS则抑制根的生长。根分生组织的细胞学分析表明，从最低剂量开始出现细胞毒性（有丝分裂指数降低）和遗传毒性（诱导细胞遗传异常和微核）。此外，氧化应激标记物的生物化学和组织化学分析提供了应激诱导的证据，特别是在最高剂量下。损伤报告可能是由于机械表面接触根外层，组织学定位证明，和纳米PS在不同的细胞隔室，在TEM下观察到的内化。目前的研究强调了纳米PS的危险性，因为它们能够内化到作物中，可以进入食物链的不同营养级。
With the aim to investigate the mechanisms of action of nano plastics (nano PS) on plants, seeds of Allium cepa were germinated for 72 h in the presence of polystyrene nano PS (50 nm size, at concentrations of 0.01, 0.1 and 1 g L-1) and, subsequently, roots were analysed by a multifaceted approach. No effect was induced by any concentration of nano PS on the percentage of seed germination while root growth was inhibited by 0.1 and 1 g L-1 nano PS. Cytological analysis of the root meristems indicated cytotoxicity (reduction of mitotic index) and genotoxicity (induction of cytogenetic anomalies and micronuclei) starting from the lowest dose. Moreover, the biochemical and histochemical analysis of oxidative stress markers gave evidence of stress induction, especially at the highest doses. Damages reported could be due to mechanical surface contact in root external layers, as evidenced by histological localization, and to the internalization of nano PS in different cellular compartments, observed under TEM. The present research underlines the hazardous nature of nano PS, that for their ability to be internalized into crop plants, can enter into different trophic levels of the food chain.