Biotransformation of the Diphenyl Ether Herbicide Lactofen and Purification of a Lactofen Esterase from Brevundimonas sp LY-2
Biotransformation of the Diphenyl Ether Herbicide Lactofen and Purification of a Lactofen Esterase from Brevundimonas sp LY-2
复制标题
二苯醚除草剂乳托芬的生物转化和短波单胞菌 LY-2 乳托芬酯酶的纯化
DOI：
10.1021/jf101974y
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发表时间：
2010-09-08
影响因子：
6.1
通讯作者：
Huang, Xing
中科院分区：
文献类型：
--
作者：
Liang, Bo;Zhao, Yu-kun;Huang, Xing
二苯醚类除草剂乳酰草醚是防治阔叶杂草的常用除草剂。一旦释放到环境中，这种除草剂就会发生微生物反应。本文报道了从土壤中分离的Brevundimonas sp. LY-2菌株对乳氟芬的生物转化。该菌株降解约80%的50毫克L-1 lactofen在5天的培养瓶中。描述了除草剂在培养基中的代谢行为。结果表明，乳酸菌灵的转化途径导致形成1-（羧基）乙基-5-（2-氯-4-（三氟甲基）苯氧基）-2-硝基苯甲酸酯和乙醇。一种酯酶，它可以切割的lactofen的链烷酸侧链的正确酯键，纯化113.3倍，以6.83%的回收率均匀。研究结果表明，Brevundimonas sp. LY-2是通过酯酶催化酯键断裂来降解乳芬的。
The diphenyl ether herbicide lactofen is commonly used to control broadleaf weeds. Once released into the environment, this herbicide is subject to microbial reactions. This study describes the biotransformation of lactofen by Brevundimonas sp. LY-2 isolated from enrichment cultures inoculated with soil sample. This strain degraded about 80% of 50 mg L-1 lactofen in 5 days of incubation in flasks. The metabolic behaviors of the herbicide in the media are described. The results show a transformation pathway of lactofen by the bacterium leading to the formation of 1-(carboxy)ethyl-5-(2-chloro-4-(trifluoromethyl)phenoxy)-2-nitrobenzoate and ethanol. An esterase, which could cleave the right ester bond of the alkanoic side chain of lactofen, was purified 113.3-fold to homogeneity with 6.83% recovery. The current results suggested that Brevundimonas sp. LY-2 degraded lactofen via the ester bond cleavage catalyzed by esterase.