Exploring laccase and mediators behavior during saccharification and fermentation of steam-exploded wheat straw for bioethanol production
Exploring laccase and mediators behavior during saccharification and fermentation of steam-exploded wheat straw for bioethanol production
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DOI：
10.1002/jctb.4774
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发表时间：
2016-06-01
影响因子：
3.4
通讯作者：
Ballesteros, Mercedes
中科院分区：
文献类型：
--
作者：
Moreno, Antonio D.;Ibarra, David;Ballesteros, Mercedes
背景：漆酶是从木质纤维原料中改进生物精制过程的一个非常强大的工具。这些酶不仅被用作生物乙醇生产的预处理剂，主要用作脱木素剂，而且还被用作去除后续发酵过程中的抑制剂(主要是酚)的生物技术工具。结果：在本工作中，用朱砂拟青霉漆酶和不同的漆酶中介系统(LMS)处理汽爆麦草中的水不溶性固形物(WIS)部分，并没有降低木质素的含量，导致后续糖化过程中葡萄糖的回收率较低。与碱抽提相结合，漆酶/LMS处理对木质素脱除或糖化没有协同作用。相反，漆酶降低了麦草全浆中的可溶性酚(占总酚的95%)，改善了发酵过程中的酵母菌性能，提高了乙醇产量。结论：无论有无介体，朱砂假单胞菌漆酶对蒸爆麦草生产生物乙醇的脱木素效果不明显，但具有解毒能力。因此，需要探索新的漆酶或设计同时具有脱木素和解毒能力的漆酶，以生产主要的全球乙醇产量。(C)2015年化学工业学会
BACKGROUND: Laccases represent a very powerful tool to improve biorefining processes from lignocellulosic feedstocks. These enzymes are being investigated not only for potential use as pretreatment agents in bioethanol production, mainly as a delignifying agent, but also as a biotechnological tool for removal of inhibitors (mainly phenols) of subsequent fermentation processes.RESULTS: In this work, the treatment of the water insoluble solids (WIS) fraction from steam-exploded wheat straw with Pycnoporus cinnabarinus laccase and different laccase-mediator systems (LMS) did not decrease the lignin content, resulting in lower glucose recoveries during the subsequent saccharification. In combination with an alkaline extraction, the treatment with laccase/LMS produced no synergistic effect enhancing the delignification or saccharification of WIS. In contrast, laccase reduced the soluble phenols (95% of the total phenols identified) of the whole slurry from steam-exploded wheat straw, improving the yeast performance during the fermentation and enhancing the ethanol yields.CONCLUSIONS: The efficiency of P. cinnabarinus laccase with or without mediators as a delignifying agent on steam-exploded wheat straw for bioethanol production was not observed, whereas its detoxification ability was shown. Thus, new laccases or designing laccases with ability to delignify and detoxify simultaneously needs to be explored in order to produce major ethanol global yields. (C) 2015 Society of Chemical Industry