The effect of Curcuma longa extracted (curcumin) on the quality of cryopreserved boar semen
The effect of Curcuma longa extracted (curcumin) on the quality of cryopreserved boar semen
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DOI：
10.1111/asj.12395
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发表时间：
2015-09-01
影响因子：
2
通讯作者：
Kaeoket, Kampon
中科院分区：
文献类型：
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作者：
Chanapiwat, Panida;Kaeoket, Kampon
本研究的目的是确定猪精液冷冻保存所需的姜黄素的最佳浓度。从9头具有生育能力的杜洛克公猪中采集精液样品（n=9），用于常规人工授精。采集精液，根据冷冻稀释液中姜黄素的浓度（分别为0、0.125、0.25、0.50、0.75和1.0mmol/L）分为6组（A ~ F组）。精液采用传统的液氮蒸气法冷冻，保存在液氮罐中，温度为-196 ℃。储存后，将冷冻精液样品在50 ℃下解冻12秒，并评价前向运动性、活力和顶体完整性。实验结果表明，姜黄素浓度为0.25（C组）和0.50mmol/L（D组）时，前向运动率分别为33.3%和36.1%，差异有显著性（P
The aim of this study was to determine the optimal concentration of curcumin needed for cryopreservation of boar semen. Semen samples (n=9) were collected from nine Duroc boars which having proven fertility were used for routine artificial insemination. Semen samples were collected and divided into six groups (groups A-F) according to various concentrations of curcumin in freezing extender (i.e. 0, 0.125, 0.25, 0.50, 0.75 and 1.0mmol/L, respectively). The semen was frozen by traditional liquid nitrogen vapor method and stored at -196 degrees C in the liquid nitrogen tank. After storage, frozen semen samples were thawed at 50 degrees C for 12s and evaluated for progressive motility, viability and acrosome integrity. The present results indicated that the addition of curcumin at 0.25 (group C) or 0.50mmol/L curcumin (group D) yielded the higher percentage of progressive motility (33.3 and 36.1%, respectively) (P