CRISPR-Cas system: Toward a more efficient technology for genome editing and beyond
CRISPR-Cas system: Toward a more efficient technology for genome editing and beyond
复制标题
DOI：
10.1002/jcb.29140
复制
发表时间：
2019-10-01
影响因子：
4
通讯作者：
Zarredar, Habib
中科院分区：
文献类型：
--
作者：
Ahmadzadeh, Vahideh;Farajnia, Safar;Zarredar, Habib
基因组工程技术使科学家能够对DNA序列进行特异性操作，对生物医学研究具有重要意义。早期引入双链DNA断裂的方法依赖于基于蛋白质的系统。这些平台已经实现了令人着迷的进步，但所有这些都是昂贵和耗时的工程，阻止这些获得高吞吐量的应用。来自细菌的CRISPR-Cas9系统在基因靶向方面产生了相当大的兴奋。在这篇综述中，我们描述了基因靶向技术，重点是最近的策略，以提高特异性的CRISPR-Cas系统的核酸酶和非核酸酶的应用。
Genome engineering technology is of great interest for biomedical research that enables scientists to make specific manipulation in the DNA sequence. Early methods for introducing double-stranded DNA breaks relies on protein-based systems. These platforms have enabled fascinating advances, but all are costly and time-consuming to engineer, preventing these from gaining high-throughput applications. The CRISPR-Cas9 system, co-opted from bacteria, has generated considerable excitement in gene targeting. In this review, we describe gene targeting techniques with an emphasis on recent strategies to improve the specificities of CRISPR-Cas systems for nuclease and non-nuclease applications.