Immunologic studies of alveolar macrophages from patients with metastatic renal cell carcinoma.
Immunologic studies of alveolar macrophages from patients with metastatic renal cell carcinoma.
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转移性肾细胞癌患者肺泡巨噬细胞的免疫学研究。
DOI：
10.1164/ajrccm/141.5_pt_1.1256
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Ahmad,M
中科院分区：
文献类型：
--
作者：
Thomassen,MJ;Barna,BP;Wiedemann,HP;Bukowski,RM;Farmer,M;Ahmad,M
肾癌患者肺泡巨噬细胞的功能，其特征是频繁的肺转移，还没有被检查。本研究的目的是评估肾癌肺泡巨噬细胞的杀瘤反应，以确定这种活性是否受到损害。取26例正常人和16例肾癌患者的肺泡巨噬细胞和/或血单核细胞。评估肺泡巨噬细胞和单核细胞对1H-胸苷标记的肿瘤靶细胞的杀肿瘤活性。暴露于脂多糖（LPS）或重组干扰素-γ的患者肺泡巨噬细胞和单核细胞表达的杀肿瘤活性与正常Subbrain中的相当。活化的肺泡巨噬细胞识别并溶解肿瘤细胞（包括同种异体肾癌细胞），但不识别非肿瘤细胞。肺转移瘤患者的肺泡巨噬细胞和单核细胞的杀瘤反应与其他部位转移瘤患者无差异。这些结果表明，肺泡巨噬细胞从肾癌患者肺转移或不妥协，在体外，但响应于免疫调节剂与增强的杀肿瘤活性，在相同的方式做正常志愿者的肺泡巨噬细胞。
Alveolar macrophage function in patients with renal carcinoma, a disease characterized by frequent pulmonary metastases, has not been examined. The purpose of this study was to evaluate tumoricidal responses of alveolar macrophages in renal carcinoma to determine if such activity is compromised. Alveolar macrophages and/or blood monocytes were obtained from 26 normal volunteers and 16patients with renal carcinoma•. Tumoricidal activity of alveolar macrophages and monocytes was assessed against'H-thymidine-Iabeled tumor target cells. Patient alveolar macrophages and monocytes exposed to lipopolysaccharide (LPS) or recombinant interferon-gamma expressed tumoricidal activity comparable to those in normal SUbjects. Activated alveolar macrophages recognized and lysed neoplastic cells (including allogenic renal carcinoma cells), but not nonneoplastic cells. Alveolar macrophge and monocyte tumoricidal responses of patients with pulmonary metastases were not different from those of patients with metastases to other sites. These results Indicate that alveolar macrophages from patients with renal carcinoma with or without pulmonary metastases are not compromised in vifro, but respond to immunomodulators with enhanced tumoricidal activity in the same fashion as do alveolar macrophages from normal volunteers.