Disruption of Zar1 leads to arrested oogenesis by regulating polyadenylation via Cpeb1 in tilapia (Oreochromis niloticus)
Disruption of Zar1 leads to arrested oogenesis by regulating polyadenylation via Cpeb1 in tilapia (Oreochromis niloticus)
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DOI：
10.1016/j.ijbiomac.2024.129632
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发表时间：
2024-01-25
影响因子：
8.2
通讯作者：
Tao，Wenjing
中科院分区：
文献类型：
--
作者：
Yu，Miao;Zhang，Shiyi;Tao，Wenjing
卵子发生是一个复杂的过程，受包括母体基因在内的多种因素的精确协调调节。合子停滞 1 (zar1) 已被确定为卵巢特异性母体基因，对于小鼠和斑马鱼的卵母细胞到胚胎的转变和卵子发生至关重要。然而，其在其他物种中的功能仍有待阐明。本研究在尼罗罗非鱼中鉴定出zar1具有保守的C端锌指结构域。zar1在卵巢中高表达，并在I期和II期卵母细胞中特异表达。 zar1的破坏导致卵原细胞向I期卵母细胞的转变失败，并伴有体细胞凋亡。在 zar1−/− 鱼的卵巢中观察到 figla、gdf9、bmp15 和 wee2 mRNA 的下调和失败。 Cpeb1 是与 Zar1 相互作用的聚腺苷酸化所必需的基因，在 zar1−/−fish 中下调。此外，在zar1−/−鱼中观察到血清雌激素水平降低和雄激素水平升高。综上所述，zar1 似乎通过调节聚腺苷酸化和雌激素合成对罗非鱼卵子发生至关重要。我们的研究表明，Zar1在不同物种中通过相似的信号通路在性腺发育过程中具有不同的分子功能。
Oogenesis is a complex process regulated by precise coordination of multiple factors, including maternal genes. Zygote arrest 1 (zar1) has been identified as an ovary-specific maternal gene that is vital for oocyte-to-embryo transition and oogenesis in mouse and zebrafish. However, its function in other species remains to be elucidated. In the present study,zar1was identified with conserved C-terminal zinc finger domains in Nile tilapia.zar1was highly expressed in the ovary and specifically expressed in phase I and II oocytes. Disruption ofzar1led to the failed transition from oogonia to phase I oocytes, with somatic cell apoptosis. Down-regulation and failed polyadenylation offigla,gdf9,bmp15andwee2mRNAs were observed in the ovaries ofzar1−/−fish. Cpeb1, a gene essential for polyadenylation that interacts with Zar1, was down-regulated inzar1−/−fish. Moreover, decreased levels of serum estrogen and increased levels of androgen were observed inzar1−/−fish. Taken together,zar1seems to be essential for tilapia oogenesis by regulating polyadenylation and estrogen synthesis. Our study shows that Zar1 has different molecular functions during gonadal development by the similar signaling pathway in different species.