Genomic organization of H2Av containing nucleosomes in Drosophila heterochromatin.
Genomic organization of H2Av containing nucleosomes in Drosophila heterochromatin.
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DOI：
10.1371/journal.pone.0020511
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Pugh BF
中科院分区：
文献类型：
--
作者：
Zhang Z;Pugh BF
H2Av 是一种多功能组蛋白变体，在果蝇的转录、DNA 修复和染色质结构中发挥着积极和消极的作用。 H2Av 及其更广泛的同源物 H2A.Z 往往富集于基因的 5' 端，并且存在于常染色质和异染色质中。它围绕常染色质基因的组织和其他特征已在许多真核模型生物中得到描述。然而，人们对异染色质中 H2Av 核小体的组织知之甚少。在这里，我们报告了位于果蝇异染色质区域的基因和转座元件周围的单个 H2Av 核小体的特性和组织。我们比较了与常色区域中发现的相似性和差异。我们的分析表明，核小体本质上定位在DNA转座元件的反向重复序列上，例如与“1360”元件相关的元件，但本质上并不定位在逆转录转座子相关元件上。
H2Av is a versatile histone variant that plays both positive and negative roles in transcription, DNA repair, and chromatin structure in Drosophila. H2Av, and its broader homolog H2A.Z, tend to be enriched toward 5′ ends of genes, and exist in both euchromatin and heterochromatin. Its organization around euchromatin genes and other features have been described in many eukaryotic model organisms. However, less is known about H2Av nucleosome organization in heterochromatin. Here we report the properties and organization of individual H2Av nucleosomes around genes and transposable elements located in Drosophila heterochromatic regions. We compare the similarity and differences with that found in euchromatic regions. Our analyses suggest that nucleosomes are intrinsically positioned on inverted repeats of DNA transposable elements such as those related to the “1360” element, but are not intrinsically positioned on retrotransposon-related elements.