Ultrastructural organization of recombinant Marburg virus nucleoprotein:: Comparison with Marburg virus inclusions
Ultrastructural organization of recombinant Marburg virus nucleoprotein:: Comparison with Marburg virus inclusions
复制标题
DOI：
10.1128/jvi.74.8.3899-3904.2000
复制
发表时间：
2000-04-01
影响因子：
5.4
通讯作者：
Becker, S
中科院分区：
文献类型：
--
作者：
Kolesnikova, L;Mühlberger, E;Becker, S
用免疫电镜研究了表达重组马尔堡病毒（MBGV）核蛋白（NP）的HeLa细胞。结果发现，MBGV NPs组装成与粗面内质网膜紧密结合的大聚集体。对这些聚集体的进一步分析表明，NPs形成了以六边形模式排列的管状结构。在MBGV感染诱导的细胞内包涵体中检测到类似的预先形成的核衣壳模式。我们的数据表明，MBGV NP能够在不存在真实病毒基因组和其他核衣壳相关蛋白的情况下形成核衣壳样结构。
HeLa cells expressing the recombinant Marburg virus (MBGV) nucleoprotein (NP) have been studied by immunoelectron microscopy. It was found that MBGV NPs assembled into large aggregates which were in close association with membranes of the rough endoplasmic reticulum. Further analysis of these aggregates revealed that NPs formed tubule-like structures which were arranged in a hexagonal pattern, A similar pattern of preformed nucleocapsids was detected in intracellular inclusions induced by MBGV infection. Our data indicated that MBGV NP is able to form nucleocapsid-like structures in the absence of the authentic viral genome and other nucleocapsid-associated proteins.