Cloning and sequencing of the mumps virus fusion protein gene.
Cloning and sequencing of the mumps virus fusion protein gene.
复制标题
腮腺炎病毒融合蛋白基因的克隆和测序。
DOI：
10.1016/0042-6822(87)90477-6
复制
发表时间：
1987
期刊：
影响因子：
3.7
通讯作者：
Wolinsky,JS
中科院分区：
文献类型：
--
作者：
Waxham,MN;Server,AC;Goodman,HM;Wolinsky,JS
腮腺炎病毒的融合蛋白（F）基因是从受感染细胞mRNA构建的cDNA文库中克隆出来的。 F特异性质粒通过与简并寡核苷酸探针杂交来鉴定，该探针的序列是从F2蛋白的N端氨基酸序列推导出来的。确定了F基因的完整核苷酸序列。该基因长1786个核苷酸，编码一个由538个氨基酸组成的长开放阅读框。 F 蛋白具有在 Cys 和 Val 残基之间切割的 19 个氨基酸信号肽。 F0蛋白活化成成熟F1,2的裂解位点是ArgArgHisLysArg。蛋白质 C 末端附近的一段 30 个疏水氨基酸后面是几个带电氨基酸，似乎充当脂质双层中蛋白质的锚定结构域。腮腺炎病毒的F基因与副粘病毒SV-5的F基因高度相关。
The fusion protein (F) gene of mumps virus was cloned from a cDNA library constructed from infected cell mRNA. The F-specific plasmids were identified by hybridization to a degenerate oligonucleotide probe whose sequence was deduced from the N-terminal amino acid sequence of the F2protein. The complete nucleotide sequence of the F gene was determined. The gene is 1786 nucleotides long and encodes one long open reading frame of 538 amino acids. The F protein has a 19-amino acid signal peptide cleaved between Cys and Val residues. The cleavage site for activation of the F0protein into themature F1,2is ArgArgHisLysArg. A stretch of 30 hydrophobic amino acids near the C-terminus of the protein is followed by serveral charged amino acids and appears to serve as the anchoring domain for the protein in the lipid bilayer. The F gene of mumps virus is highly related to the F gene of the paramyxovirus SV-5.