Characterization of novel cytoplasmic PARP in the brain of Octopus vulgaris.
Characterization of novel cytoplasmic PARP in the brain of Octopus vulgaris.
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DOI：
10.1086/bblv222n3p176
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发表时间：
2012-06
期刊：
影响因子：
--
通讯作者：
Di Cosmo A
中科院分区：
文献类型：
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作者：
De Lisa E;De Maio A;Moroz LL;Moccia F;Mennella MR;Di Cosmo A
最近的研究主要集中在聚（adp -核糖）聚合酶（PARP）反应在无脊椎动物中枢神经系统（CNS）长期记忆（LTM）过程中的参与。在本文中，我们描述，定位，并分配一个可能的作用，细胞质PARP在章鱼的大脑。在视叶、食管上肿块和视神经中检测PARP活性。酶在细胞质部分中含量最高。在视觉识别训练后，章鱼大脑中检测到该酶的超激活。最后发现胞质PARP对章鱼肌动蛋白聚合有抑制作用。我们提出细胞质PARP在体内发挥作用，诱导学习诱导神经元可塑性过程中发生的细胞骨架重组。
Recent investigation has focused on the participation of the poly (ADP-ribose) polymerase (PARP) reaction in the invertebrate central nervous system (CNS) during the process of long-term memory (LTM). In this paper, we characterize, localize, and assign a possible role to a cytoplasmic PARP in the brain of Octopus vulgaris. PARP activity was assayed in optic lobes, supraesophageal mass, and optic nerves. The highest levels of enzyme were found in the cytoplasmic fraction. Hyper-activation of the enzyme was detected in Octopus brain after visual discrimination training. Finally, cytoplasmic PARP was found to inhibit Octopus vulgaris actin polymerization. We propose that the cytoplasmic PARP plays a role in vivo to induce the cytoskeletonal reorganization that occurs during learning-induced neuronal plasticity.