Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice
Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice
复制标题
灭活肺炎链球菌作为非致病性颗粒对小鼠呼吸道合胞病毒感染肺炎严重程度的影响
DOI：
10.1016/j.toxrep.2019.05.004
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Kurokawa,
中科院分区：
文献类型：
--
作者：
Miyauchi;A.;Watanabe;W.;Akashi;T.;Hashiguchi;S.;Yoshida;H.;Sugita;C.;Kurokawa,
呼吸道合胞病毒（RSV）感染肺炎的严重程度与宿主免疫反应和外部因素（如细菌和环境化学物质）密切相关。我们研究了灭活的肺炎链球菌（ISP）作为非致病性颗粒对RSV感染小鼠肺炎严重程度的影响。在RSV感染之前，小鼠鼻内暴露于ISP。在感染后第5天，我们检查了肺中的组织、病毒滴度和浸润细胞。ISP在RSV感染小鼠的肺中不引起显著的组织病理学效应，但降低病毒滴度。肺内淋巴细胞浸润率降低，巨噬细胞浸润率升高。此外，我们发现ISP在感染后第1天增加RSV感染小鼠支气管肺泡灌洗液中RANTES水平，但降低I型干扰素水平。因此，ISP不会加重RSV感染的肺炎，相反，它可能会轻度降低严重程度。我们表征并讨论了ISP作为非致病性颗粒诱导RANTES对RSV感染中肺炎的作用的固有活性。
The severity of pneumonia in respiratory syncytial virus (RSV) infection is strongly related to host immune response and external factors such as bacteria and environmental chemicals. We investigated the effect of inactivatedStreptococcus pneumoniae(ISP) as non-pathogenic particles on the severity of pneumonia in RSV-infected mice. Mice were intranasally exposed to ISP before RSV infection. On day 5 post-infection, we examined tissues, virus titer, and infiltrated cells in the lungs. The ISP did not cause significant histopathological effects in the lungs of RSV infected mice, but reduced virus titer. It also reduced the ratio of lymphocyte infiltration into the lungs and consequently the ratio of macrophage increased. In addition, we found that ISP increased RANTES level in bronchoalveolar lavage fluid from RSV-infected mice on day 1 post-infection, but reduced type I interferon levels. Thus, ISP did not exacerbate pneumonia in RSV infection, rather, it might mildly reduce the severity. We characterize and discuss the inherent activity of ISP as non-pathogenic particles inducing the role of RANTES on the pneumonia in RSV infection.