Measurements of tissue blood flow by the hydrogen clearance technique (HCT): A comparative study including laser Doppler flowmetry (LDF) and the erlangen micro‐lightguide spectrophotometer (EMPHO)
Measurements of tissue blood flow by the hydrogen clearance technique (HCT): A comparative study including laser Doppler flowmetry (LDF) and the erlangen micro‐lightguide spectrophotometer (EMPHO)
复制标题
通过氢清除技术（HCT）测量组织血流量：激光多普勒血流计（LDF）和埃尔兰根微光导分光光度计（EMPHO）的比较研究
作者：
H. Machens;Norbert Pallua;P. Mailaender;J. Pasel;K. Frank;R. Reimer;A. Berger
本研究的目的是将氢清除技术 (HCT) 与两种不同的成熟技术（即激光多普勒流量计 (LDF) 和 Eriangen 微光导分光光度计 (EMPHO)）进行比较，用于在实验环境中测量组织血流。对于动物实验，我们选择了使用上腹腹股沟皮瓣的动脉和静脉皮瓣血栓形成大鼠模型。该研究纳入了 40 只雄性深色 Aguty 大鼠。这些动物被分为八组，每组都有不同的血管血栓模型。 HCT 用于收集 1,467 个测量值，并记录 2,934 个图表；由于电极放置错误和电子噪声，27 项测量（54 个图表）不得不被丢弃。在 27 次丢弃的测量中，有 19 次失败的原因是清醒动物的移动破坏了衰变信号。 LDF 和 EMPHO 测量在每个测量阶段连续进行。使用简单和多元线性回归以及配对 t 检验来比较这三种技术。第 1、3、6 和 7 相中记录的血流值的再现性在 7.8% 至 13.6% 之间变化，与 LDF 和 EMPHO 的范围大致相同。在这方面我们找不到这三种技术之间的显着差异。 HCT 和 LDF 的相关系数为 r = 0.89。对于 HCT 和 EMPHO，我们发现 r = 0.67。 HCT 检测动脉和静脉瓣血栓形成的敏感性和特异性均为 1.0；对于 LDF，我们发现值分别为 0.89 和 0.92。 EMPHO 的敏感性和特异性值分别为 0.92 和 0.95。在对用于测量局部组织血流的三种不同技术（HCT、LDF 和 EMPHO）进行仔细评估后，我们得出的结论是，HCT 必须作为定量测量局部组织血流和早期诊断动静脉瓣血栓形成的可靠工具。 © 1995 Wiley-Liss, Inc.
The purpose of this study was to compare the hydrogen clearance technique (HCT) with two different, well‐established techniques, i.e., the laser Doppler flowmetry (LDF) and the Eriangen micro‐lightguide spectrophotometer (EMPHO), for tissue blood flow measurements in an experimental setting. For the animal experiments, we chose a rat model for arterial and venous flap thrombosis, using the epigastric groin flap. Forty male dark Aguty rats were included in the study. The animals were divided into eight groups, each with a different vascular thrombotic model. HCT was used to collect 1,467 measurements, and 2,934 graphs were recorded; 27 measurements (54 graphs) had to be discarded due to faulty electrode placements and electronic noise. In 19 of the 27 discarded measurements the cause of failure was moving of the awaking animal with disruption of the decay signal. The LDF and EMPHO measurements were performed continuously during each measuring phase. Simple and multiple linear regression and paired t‐tests were used to compare the three techniques. The reproducibility of registered blood flow values in phases 1, 3, 6 and 7 varied between 7.8% and 13.6% which is in about the same range as LDF and EMPHO. We could not find a significant difference between the three techniques in this regard. The correlation coefficient for HCT and LDF was r = 0.89. For HCT and EMPHO we found r = 0.67. Sensitivity and specificity values for HCT were both 1.0 in detecting arterial and venous flap thrombosis; for LDF we found values of 0.89 and 0.92, respectively. Sensitivity and specificity values for EMPHO were 0.92 and 0.95, respectively. After careful evaluation of three different techniques (HCT, LDF, and EMPHO) for measurements of local tissue blood flow we came to the conclusion that HCT must be favoured as a reliable tool for quantitative measurement of local tissue blood flow and early diagnosis of arterial and venous flap thrombosis. © 1995 Wiley‐Liss, Inc.