A detection method for recombinant DNA from genetically modified soybeans and processed foods containing them
A detection method for recombinant DNA from genetically modified soybeans and processed foods containing them
复制标题
转基因大豆及其加工食品中重组DNA的检测方法
DOI：
10.3358/shokueishi.40.149
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
A. Hino
中科院分区：
文献类型：
--
作者：
T. Matsuoka;Y. Kawashima;H. Akiyama;H. Miura;Y. Goda;T. Sebata;K. Isshiki;M. Toyoda;A. Hino
采用聚合酶链反应（PCR）技术，对从美国进口的转基因大豆（GMS）与传统非转基因大豆（non-GMS）混合后的食品或食品成分进行检测。根据外源基因的序列设计了3对特异性寡核苷酸引物，可检测到大豆中重组脱氧核糖核酸（DNA）的存在。大豆内源凝集素Le 1基因被用作内部对照。PCR扩增结果表明，十六烷基三甲基溴化铵（CTAB）法最适合大豆及其加工食品中DNA的提取。在含有0.05%GMS的干大豆和含有0.5%GMS的大豆制成的豆腐中可以检测到重组DNA。在41个市售豆腐样品中，27个样品中检测到重组DNA。然而，尽管当采用巢式PCR技术时，从在115° C蒸煮的大豆和发酵纳豆中检测到Le 1基因，但是难以对从在131° C蒸煮的大豆或发酵纳豆中提取的DNA进行PCR。
A method using polymerase chain reaction (PCR) was designed for the detection of food or food ingredients derived from genetically modified soybeans (GMS), imported from the United States, in a mixture with conventional non-genetically modified soybeans (non-GMS). The presence of recombinant deoxyribonucleic acid (DNA) in the soybeans could be detected with three different pairs of specific oligonucleotide primers designed from the sequences of the introduced genes. The soybean intrinsic lectin Le1 gene was used as an internal control. The results of the PCR amplification indicated that a method using cetyltrimethylammonium bromide (CTAB) was most suitable for DNA extraction from soybeans and the processed foods. The recombinant DNA could be detected in dry soybeans containing 0.05% GMS and tofu made from soybeans containing 0.5% GMS. Of 41 commercial tofu samples, recombinant DNA was detected from 27 tofu samples. It is, however, difficult to carry out PCR on DNA extracted from soybeans steamed at 131°C or on fermented natto, although the Le1 gene was detected from soybeans steamed at 115°C and in the fermented natto when a nested PCR technique was employed.