Transcriptional regulation of uncoupling protein-2 gene expression in L6 myotubes.
Transcriptional regulation of uncoupling protein-2 gene expression in L6 myotubes.
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L6 肌管中解偶联蛋白 2 基因表达的转录调控。
DOI：
10.1038/sj.ijo.0801812
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Scarpace,PJ
中科院分区：
文献类型：
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作者：
Hatakeyama,Y;Scarpace,PJ
目的：研究维甲酸X受体(RXR)的全反式维甲酸(Tra)和多不饱和脂肪酸和过氧化物酶体增殖物激活受体(PPAR)配体亚麻酸对培养的L 6肌管UCP2基因表达的影响，以加深对骨骼肌细胞UCP2基因转录调控的认识。斑点印迹定量分析反式亚麻酸和亚麻酸对UCP2基因表达的时间和浓度依赖性。结果：TrA以时间和浓度依赖的方式诱导α，γ和δ基因表达。与trA类似，0.5 mM亚麻酸可显著增加UCP2mRNA的表达。结论：反式亚麻酸和亚麻酸可上调δ在L 6肌管中的表达，其机制可能与PPAR和RXRs有关。
OBJECTIVE: To increase the understanding of the transcriptional regulation of UCP2 gene expression in skeletal muscle cells, we examined the effect of all-trans-retinoic acid (tRA), a ligand (after the conversion to 9-cis-RA) of the retinoid X receptor (RXR), and linolenic acid, a polyunsaturated fatty acid and peroxisome proliferator-activated receptors (PPARs) ligand, on the expression of UCP2 mRNA in cultured L 6 myotubes.RESEARCH METHODS AND PROCEDURES: UCP2 gene expression in L 6 myotubes was confirmed by Northern blot analysis. The time-and concentration-dependency of tRA and linolenic acid on UCP2 gene expression was assessed by dot blot quantification. The mRNA levels of PPAR subtypes (α, γ and δ) were determined by RT-PCR.RESULTS: tRA induced UCP2 gene expression in a time-and concentration-dependent manner. Similar to tRA, UCP2 mRNA was markedly increased by 0.5 mM linolenic acid. In L 6 myotubes, PPARδ mRNA was abundant, whereas PPARα mRNA was lower and PPARγ mRNA was minimal.CONCLUSIONS: UCP2 mRNA expression in L 6 myotubes is up-regulated by tRA and linolenic acid, possibly through a mechanism involving PPAR and RXRs.