Nicotine induced changes in gene expression by human coronary artery endothelial cells
Nicotine induced changes in gene expression by human coronary artery endothelial cells
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DOI：
10.1016/s0021-9150(00)00475-5
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发表时间：
2001-02-01
期刊：
影响因子：
5.3
通讯作者：
Ye, S
中科院分区：
文献类型：
--
作者：
Zhang, SL;Day, I;Ye, S
The primary role of cigarette smoking in the development of coronary heart disease is to cause damage to the vascular endothelium, leading to endothelial cell dysfunction and initiating the pathogenesis of coronary atherosclerosis. We studied the response of human coronary artery endothelial cells to nicotine exposure by examining the expression of a panel of genes encoding molecules that have been shown to be involved ill atherogenesis. Treatment of primary human coronary artery endothelial cells with nicotine for 24 h at concentrations (10(-5) and 10(-7) M) similar to those in the blood of smokers resulted in increased mRNA levels of endothelial nitric oxide synthase, angiotensin-1 converting enzyme, tissue-type plasminogen activator. plasminogen activator inhibitor-1, von Willebrand factor, and vascular cell adhesion molecule-1. No change was detected in the expression levels of the genes encoding basic fibroblast growth factor, endothelin-l. endothelial leukocyte adhesion molecule-1 and matrix metalloproteinase-2 Finder these conditions. These data indicate that nicotine alters the expression of a number of endothelial genes whose products play major roles in regulating the vascular tone and thrombogenicity, making a contribution to the understanding of the effects of cigarette smoking on the development of coronary atherosclerosis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
