Quantitative analysis of oxytetracycline and related substances by high-performance liquid chromatography.

Quantitative analysis of oxytetracycline and related substances by high-performance liquid chromatography.
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高效液相色谱法定量分析土霉素及有关物质。

DOI：
10.1016/s0021-9673(01)81765-9
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发表时间：
1987
期刊：
Journal of chromatography
影响因子：
--
通讯作者：
H. Vanderhaeghe
H. Vanderhaeghe
中科院分区：
--
文献类型：
--
作者：
N. Khan;E. Roets;J. Hoogmartens;H. Vanderhaeghe

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Isocratic high-performance liquid chromatography onPLRP-S8-μm poly(styrene-divinylbenzene) copolymer allows complete separation of oxytetracy-cline, 4-epioxytetracycline, tetracycline, anhydrooxytetracycline, α- and β-apooxytetracycline. The mobile phase wastert.-butanol0.2Mphosphate buffer pH8.00.02 M tetrabutylammonium sulphate pH80.0001Msodium ethylenediaminetetraacetate pH8.0-water (5.9:10:5:10:78.1, m/v/v/v/v). With this isocratic method, 2-acetyl-2-decarboxamidooxytetracycline is only partly resolved from oxytetracycline. The separation and the detection limits can be improved by the use of gradient elution. Gradient elution was used for the comparison of official standards and for the analysis of a number of commercial samples, and to monitor the stability of oxytetracycline hydrochloride during storage in the solid state for about 6 years at various temperatures.