Disposable electrochemical immunosensor for simultaneous assay of a panel of breast cancer tumor markers
Disposable electrochemical immunosensor for simultaneous assay of a panel of breast cancer tumor markers
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用于同时测定一组乳腺癌肿瘤标志物的一次性电化学免疫传感器
DOI：
10.1039/c2an35967g
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Chen, Dairong
中科院分区：
文献类型：
--
作者：
Ge, Shenguang;Yu, Feng;Chen, Dairong
采用丝网印刷技术在含有三个石墨工作电极的碳电极阵列上制备了多路复用免疫传感器阵列。该免疫传感器是利用石墨烯（GR）修饰免疫传感器表面以加速电子转移而实现的。在GR修饰的电极表面原位合成AuNPs，目的是固定化捕获抗体Ab(1)。通过夹心式免疫反应，在免疫传感器表面捕获碱性磷酸酶（ALP）标记的抗体（Ab(2)）功能化Au簇(auc)/GR，催化底物中3-吲哚酚磷酸水解，生成吲哚酚中间体还原Ag离子。ALP和aus /GR同时催化银沉积过程，使检测信号放大。然后在KCl溶液中采用阳极线性扫描伏安溶出法测定银的沉积量。剥离峰值电流的增加成正比的对数价值CA 153 CA 125和CEA浓度范围5.0 x 10 (3) 50 U为CA 153毫升(1),1.0 x 10(3)为CA 100 U mL(1) 125年,和4.0 x 10 (3) 200 ng毫升(1)东航,限制检测的UmL 1.5 x 10 (3) (1) 153 CA, UmL 3.4 x 10 (4) (1) CA 125和1.2 x 10毫升(1)(3)ng CEA。该一次性免疫传感器阵列和简单的检测方法可快速测量一组肿瘤标志物，避免了电化学免疫分析的串扰和脱氧需要，在癌症筛查中具有重要的临床应用价值，为便捷的即时检测和商业应用提供了巨大的潜力。
The multiplexed immunosensor array was fabricated on a carbon electrode array containing three graphite working electrodes, which was prepared with screen-printed technology. This immunosensor was achieved using graphene (GR) to modify the immunosensor surface for accelerating electron transfer. The in situ synthesis of AuNPs on GR modified electrode surface aimed at the immobilization of capture antibody (Ab(1)). With a sandwich-type immunoreaction, the alkaline phosphatase (ALP)labeled antibody (Ab(2)) functionalized Au cluster (AuCs)/GR was captured on the immunosensor surface to catalyze the hydrolysis of 3-indoxyl phosphate in substrate, which produced an indoxyl intermediate to reduce Ag ion. The silver deposition process was catalyzed by both ALP and AuCs/GR, which amplified the detection signal. The deposited silver was then measured by anodic linear sweep voltammetric stripping analysis in KCl solution. The increase of stripping peak currents was proportional to the logarithm value of the CA 153, CA 125 and CEA concentration in the range 5.0 x 10(-3) to 50 U mL(-1) for CA 153, 1.0 x 10(-3) to 100 U mL(-1) for CA 125, and 4.0 x 10(-3) to 200 ng mL(-1) for CEA, with a limit detection of 1.5 x 10(-3) UmL(-1) for CA 153, 3.4 x 10(-4) UmL(-1) for CA 125, and 1.2 x 10(-3) ng mL(-1) for CEA. The disposable immunosensor array and simple detection method for fast measurement of a panel of tumor markers avoided cross-talk and the need for deoxygenation for the electrochemical immunoassay, and showed significant clinical value for application in cancer screening, providing great potential for convenient point-of-care testing and commercial application.