Dose-dependent effects of caffeine in human Sertoli cells metabolism and oxidative profile: Relevance for male fertility
Dose-dependent effects of caffeine in human Sertoli cells metabolism and oxidative profile: Relevance for male fertility
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DOI：
10.1016/j.tox.2014.12.003
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发表时间：
2015-02-03
期刊：
影响因子：
4.5
通讯作者：
Oliveira, Pedro F.
中科院分区：
文献类型：
--
作者：
Dias, Tania R.;Alves, Marco G.;Oliveira, Pedro F.
咖啡因是一种广泛消费的物质，存在于几种饮料中。育龄青年人对富含咖啡因的能量饮料的消费量越来越大。咖啡因被描述为细胞代谢的调节剂。因此，我们假设它改变了人类支持细胞（hSCs）的代谢和氧化谱，这是精子发生所必需的。为此目的，用增加剂量的咖啡因（5、50、500 μ M）培养hSC。最低浓度（5和50 μ M）的咖啡因刺激乳酸产生，但只有暴露于50 μ M的hSC显示葡萄糖转运蛋白（GLUT）的表达增加。在最高浓度（500 μ M），咖啡因刺激LDH活性，以维持乳酸生产。值得注意的是，hSCs的抗氧化能力以剂量依赖性方式降低，暴露于500 μ M咖啡因的SCs呈现促氧化潜力，同时增加蛋白质氧化损伤。因此，适量摄入咖啡因似乎对男性生殖健康是安全的，因为它刺激SC产生乳酸，这可以促进生殖细胞的存活。然而，大量饮用补充有咖啡因的能量饮料和食物应谨慎，以避免对hSCs功能的有害影响，从而避免异常精子发生。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Caffeine is a widely consumed substance present in several beverages. There is an increasing consumption of energetic drinks, rich in caffeine, among young individuals in reproductive age. Caffeine has been described as a modulator of cellular metabolism. Hence, we hypothesized that it alters human Sertoli cells (hSCs) metabolism and oxidative profile, which are essential for spermatogenesis. For that purpose, hSCs were cultured with increasing doses of caffeine (5, 50, 500 mu M). Caffeine at the lowest concentrations (5 and 50 mu M) stimulated lactate production, but only hSCs exposed to 50 mu M showed increased expression of glucose transporters (GLUTs). At the highest concentration (500 mu M), caffeine stimulated LDH activity to sustain lactate production. Notably, the antioxidant capacity of hSCs decreased in a dose-dependent manner and SCs exposed to 500 mu M caffeine presented a pro-oxidant potential, with a concurrent increase of protein oxidative damage. Hence, moderate consumption of caffeine appears to be safe to male reproductive health since it stimulates lactate production by SCs, which can promote germ cells survival. Nevertheless, caution should be taken by heavy consumers of energetic beverages and food supplemented with caffeine to avoid deleterious effects in hSCs functioning and thus, abnormal spermatogenesis. (C) 2014 Elsevier Ireland Ltd. All rights reserved.