QTL Analysis of Seed Coat Cracking in Soybean
QTL Analysis of Seed Coat Cracking in Soybean
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DOI：
10.2135/cropsci2009.08.0440
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发表时间：
2010-07-01
期刊：
影响因子：
2.3
通讯作者：
Takahashi, Ryoji
中科院分区：
文献类型：
--
作者：
Oyoo, Maurice E.;Benitez, Eduardo K.;Takahashi, Ryoji
大豆[Glycine max（L.）在现场条件下出现裂纹。本研究旨在利用DNA分子标记技术研究油菜种皮开裂的遗传基础。重组自交系（RILs）是从一个敏感的cv。那须代罗姆和一个宽容的cv。恩丽在田间种植了95个F-6 RIL（2006）和92个F-7 RIL（2007），平均每株系24株植物。花后40 d去除上部50%的豆荚可促进种皮开裂。通过开裂指数（未开裂，0至严重开裂，4）对每个种子的开裂程度进行评分。对每株植物和随后的每株系的开裂指数取平均值（平均开裂指数，Ad）。利用114个简单重复序列标记和一个形态性状进行了连锁作图。这些标记与18个连锁群相关联，跨度为1232 cM。在分子连锁群（MLG）D1 b和MLG M中分别检测到两个数量性状位点（QTL）cr 1和cr2。cr2 OIL仅在F6代中检测到，而cr 1则在不同年份和世代中检测到。cr 1的比值分数的对数为4.78（F-6）和4.77（F-7），占表型变异的27.2%（F-6）和23.5%（F-7）。在F-8近等基因系中，cr 1与种子大小或成熟度无关。因此，QTL周围的标记可用于育种以减少开裂症状而不改变种子大小或成熟度。
The seed coat of soybean [Glycine max (L.) Merr] cracks under field conditions. This study was conducted to investigate the genetic basis of seed coat cracking using DNA markers. Recombinant inbred lines (RILs) were developed from a cross between a sensitive cv. Nasushirome and a tolerant cv. Enrei. Ninety-five F-6 RILs (2006) and 92 F-7 RILs (2007) with an average of 24 plants per line were grown in the field. Seed coat cracking was promoted by removing the upper 50% of pods at 40 d after anthesis. The degree of cracking of each seed was scored by a cracking index (not cracked, 0, to severely cracked, 4). Cracking index was averaged per plant and, subsequently, per line (average cracking index, Ad). Linkage mapping was performed using 114 simple sequence repeat markers and one morphological trait. The markers were associated with 18 linkage groups spanning 1232 cM. Two quantitative trait loci (QTL) for Ad, cr1 in molecular linkage group (MLG) D1b and cr2 in MLG M, were identified. The cr2 OIL was detected only in the F6 generation, whereas cr1 was identified across years and generations The cr1 had a logarithm of odds score of 4.78 (F-6) and 4.77 (F-7), accounting for 27.2 (F-6) and 23.5% (F-7) of phenotypic variation. The cr1 was not associated with seed size or maturity in F-8 near-isogenic lines. Therefore, markers around the QTL may be useful for breeding to reduce cracking symptoms without altering seed size or maturity.