MicroRNA-212 targets SIRT2 to influence lipogenesis in bovine mammary epithelial cell line
MicroRNA-212 targets SIRT2 to influence lipogenesis in bovine mammary epithelial cell line
复制标题
MicroRNA-212靶向SIRT2影响牛乳腺上皮细胞系的脂肪生成
DOI：
10.1017/s0022029920000229
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发表时间：
2020-05-01
影响因子：
2.1
通讯作者：
Yang, Zhangping
中科院分区：
文献类型：
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作者：
Lu, Xubin;Xia, Hailei;Yang, Zhangping
在本研究中，我们筛选并验证了可能靶向SIRT2基因的mirna，并描述了miRNA-212可能通过靶向SIRT2调控乳腺上皮细胞系脂质基因的机制。生物信息学分析显示，牛SIRT2基因受miR-212、miR-375和miR-655三个mirna调控。通过qRT-PCR、western blot和荧光素酶多重验证技术对这三个mirna进行了验证和筛选，只有miR-212被证明与SIRT2具有靶向关系。miR-212与沉默RNA （siRNA）共转染的结果显示，miR-212可以通过靶向SIRT2调控脂肪酸合成酶（FASN）和甾醇调节元件结合因子1 （SREBP1）的表达，但不能调控过氧化物酶体增殖因子激活受体γ (PPAR γ)的表达。甘油三酯（TAG）含量的测定表明，miR-212增加了乳腺上皮细胞系的脂肪含量。研究表明，miR-212可以靶向并抑制SIRT2基因的表达，促进乳腺上皮细胞系的脂肪生成。
In this research paper we filter and verify miRNAs which may target silent information regulator homolog 2 (SIRT2) gene and then describe the mechanism whereby miRNA-212 might regulate lipogenic genes in mammary epithelial cell lines via targeting SIRT2. Bioinformatics analysis revealed that the bovine SIRT2 gene is regulated by three miRNAs: miR-212, miR-375 and miR-655. The three miRNAs were verified and screened by qRT-PCR, western blot, and luciferase multiplex verification techniques and only miR-212 was shown to have a targeting relationship with SIRT2. The results of co-transfecting miR-212 and silencing RNA (siRNA) showed that by targeting SIRT2, miR-212 can regulate the expression of fatty acid synthetase (FASN) and sterol regulatory element binding factor 1 (SREBP1) but not peroxisome proliferator-activated receptor gamma (PPAR gamma). Measurement of triglyceride (TAG) content showed that miR-212 increased the fat content of mammary epithelial cell lines. The study indicates that miR-212 could target and inhibit the expression of the SIRT2 gene to promote lipogenesis in mammary epithelial cell lines.