Use of chromatofocusing to detect a transferrin variant in serum of alcoholic subjects.
Use of chromatofocusing to detect a transferrin variant in serum of alcoholic subjects.
复制标题
使用色谱聚焦检测酒精受试者血清中的转铁蛋白变异体。
DOI：
10.1093/clinchem/31.9.1543
复制
发表时间：
1985
影响因子：
9.3
通讯作者：
J. Halliday
中科院分区：
文献类型：
--
作者：
E. Storey;U. Mack;L. Powell;J. Halliday
我们描述了一种检测血清中异常（pl 5.7）转铁蛋白成分的技术，这种成分在长时间大量饮酒后出现。用DEAE-Affi-Gel Blue层析纯化血清转铁蛋白，用离子交换柱（Mono P）进行色谱聚焦分析。20例有饮酒史或长期饮酒（每天100 g）的患者中有17例（85%）检测到转铁蛋白成分异常，对照组每天饮酒达80 g，持续7天，但14例正常对照组或14例与酒精无关的肝病患者未检测到转铁蛋白成分异常。在停止饮酒后的三周内，血清中的这种变异逐渐消失。它显然是由普通人转铁蛋白的脱氮作用产生的。我们发现离子交换柱上的色谱聚焦是一种敏感可靠的鉴定技术，并得出结论，检测到这种脱盐的转铁蛋白表明最近长期饮酒。
We describe a technique for detecting an abnormal (pl 5.7) transferrin component in serum, which appears after prolonged heavy consumption of alcohol. Serum transferrin was purified by chromatography on DEAE-Affi-Gel Blue and analyzed by chromatofocusing on an ion-exchange column (Mono P). The abnormal transferrin component was detected in 17 of 20 patients (85%) with a history or prolonged consumption of alcohol (100 g per day), and in control subjects who ingested up to 80 g of alcohol per day for seven days, but not in 14 normal control subjects or 14 patients with liver disease unrelated to alcohol. The variant consistently disappeared from the serum within three weeks of cessation of alcohol consumption. It is apparently produced by desialylation of ordinary human transferrin. We find that chromatofocusing on an ion-exchange column is a sensitive and reliable technique for its identification and conclude that detection of this desialylated transferrin indicates recent prolonged alcohol ingestion.