Bovine cardiac mitochondrial ADP/ATP-carrier: two distinct mRNAs and an unusually short 3'-noncoding sequence.
Bovine cardiac mitochondrial ADP/ATP-carrier: two distinct mRNAs and an unusually short 3'-noncoding sequence.
复制标题
牛心脏线粒体 ADP/ATP 载体：两种不同的 mRNA 和异常短的 3 非编码序列。
DOI：
10.1016/s0006-291x(86)80574-5
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发表时间：
1986
影响因子：
3.1
通讯作者：
Wohlrab,H
中科院分区：
文献类型：
--
作者：
Rasmussen,UB;Wohlrab,H
牛心肌线粒体ADP/ATP载体的cDNA克隆已被分离。它们与两种大小相差约300个核苷酸的mRNA杂交。两种mRNA的浓度比和总丰度在牛组织（心脏、肝脏、肾脏和子宫）中变化。其中至少有一个具有异常短的3 ′非编码序列，包括两个共有切割/多聚腺苷酸化信号序列CATTG和ATTAAA。3 ′-端显示与人U4小核RNA的区域互补。预测的氨基酸序列证实了报告的从Val 207到C-末端Val 297的序列，已提出（残基279 - 291）有助于核苷酸结合位点。
cDNA clones for bovine cardiac mitochondrial ADP/ATP carrier have been isolated. They hybridize with two mRNAs that differ in size by about 300 nucleotides. The concentration ratio and total abundance of the two mRNAs varies among bovine tissues (heart, liver, kidney, and uterus). At least one of them has an unusually short 3′-noncoding sequence, which includes the two consensus cleavage/polyadenylation signal sequences CATTG and ATTAAA. The 3′-end shows complementarity to regions of human U4 small nuclear RNA. The predicted amino acid sequence confirms the reported sequence from Val207to the C-terminal Val297, which has been proposed (residue 279–291) to contribute to a nucleotide binding site.