Factors from paraventricular nucleus mediating adrenocorticotropin release in cats.
Factors from paraventricular nucleus mediating adrenocorticotropin release in cats.
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室旁核介导猫促肾上腺皮质激素释放的因素。
DOI：
10.1152/ajpregu.1987.252.1.r109
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Gann,DS
中科院分区：
文献类型：
--
作者：
Carlson,DE;Gann,DS
实验在α-氯醛糖-β-麻醉的猫中进行。我们在侧脑室（icv）注射精氨酸加压素（AVP）、促肾上腺皮质激素释放因子（CRF）和正常兔血清（NRS）的抗血清之前和之后电刺激下丘脑室旁核（PVH）。刺激背侧PVH的腹侧部分导致动脉压和心率增加，但在任何icv治疗后均未发生变化。然而，每种药物对刺激后血浆促肾上腺皮质激素（ACTH）增加的影响与刺激的PVH面积有关。从PVH前部向背侧延伸的吻侧区对刺激的反应被抗AVP完全阻断，但不被抗CRF或NRS阻断。对刺激位于背侧PVH的尾侧区域的反应在抗CRF后减弱，在抗AVP后不变，在NRS后增强。免疫细胞化学发现icv注射的抗体进入正中隆起的部位，包括一些邻近门静脉的部位。免疫细胞化学定位的AVP-和CRF的神经元在PVH显示，PVH的前部有最高比例的AVP神经元的PVH，但只有少数CRF神经元。相反，背侧PVH含有最高密度的CRF神经元以及一些AVP神经元。我们认为，在猫，前PVH的主要释放因子是AVP，背PVH的主要释放因子是CRF。
Experiments were conducted in alpha-chloralose-urethan-anesthetized cats. We stimulated the hypothalamic paraventricular nucleus (PVH) electrically before and after intracerebroventricular (icv) injection of an antiserum to arginine vasopressin (AVP), one to corticotropin-releasing factor (CRF), or one to normal rabbit serum (NRS). Stimulation of the ventral portion of the dorsal PVH led to increases in arterial pressure and heart rate that did not change after any of the icv treatments. However, the effect of each agent on the increases in plasma adrenocorticotropin (ACTH) after stimulation was related to the area of the PVH that was stimulated. The response to stimulation of a rostral area that extended dorsally from the anterior PVH was blocked completely by the anti-AVP but not by anti-CRF or NRS. The response to stimulation of a caudal area located in the dorsal PVH was attenuated after anti-CRF, unchanged after anti-AVP, and augmented after NRS. The antibodies that were given icv were found immunocytochemically to enter the median eminence at sites that include some adjacent to the portal vessels. Immunocytochemical localization of AVP- and of CRF-containing neurons in the PVH showed that the anterior PVH had the highest proportion of AVP neurons in the PVH but had only a few CRF neurons. In contrast, the dorsal PVH contained the highest density of CRF neurons in the PVH as well as some AVP neurons. We suggest that, in the cat, the primary releasing factor for the anterior PVH is AVP and that for the dorsal PVH is CRF.