Mau-2 acts cell-autonomously to guide axonal migrations in Caenorhabditis elegans
Mau-2 acts cell-autonomously to guide axonal migrations in Caenorhabditis elegans
复制标题
DOI：
10.1242/dev.01433
复制
发表时间：
2004-12-01
期刊：
影响因子：
4.6
通讯作者：
Hekimi, S
中科院分区：
文献类型：
--
作者：
Bénard, CY;Kébir, H;Hekimi, S
在线虫的发育过程中，MAU-2基因被发现是引导细胞和轴突沿前后和背腹体轴线迁移所必需的。我们发现MAU-2编码一种新的，以前没有特征的蛋白质，在动物中高度保守。母体MAU-2基因的表达足以维持正常发育，直到第四幼虫阶段，MAU-2：：GFP融合蛋白定位于神经元的细胞质。MAU-2在原肠发育后期的胚胎中普遍表达，随着形态发生的进展，MAU-2主要在神经系统中表达。MAU-2在单个神经元中的表达挽救了MAU-2突变体的导向缺陷，表明MAU-2具有细胞自主功能。改变背向排斥剂SLT-1和MAU-2的活性导致AVM轴突的背侧投射异常，这是一种新的表型，表明MAU-2通过SLT-1不依赖的机制参与AVM的引导。综上所述，MAU-2定义了一种新的指导因子，它可能参与迁移细胞和轴突在发育过程中遇到的指导线索的细胞内处理。
The gene mau-2 has been found to be required for the guidance of cellular and axonal migrations along both the anteroposterior and the dorsoventral body axes during the development of the nematode C elegans. We show that mau-2 encodes a novel, previously uncharacterized protein that is highly conserved among animals. Maternal mau-2 gene expression is sufficient for normal development until the fourth larval stage, and a MAU-2::GFP fusion protein localizes to the cytoplasm of neurones. mau-2 is ubiquitously expressed in embryos by late gastrulation and becomes predominantly expressed in the nervous system as morphogenesis progresses. Expression of mau-2 within individual neurones rescues the guidance defects of mau-2 mutants, indicating that mau-2 functions cell-autonomously. Altering the activity of both the dorsal repellent slt-1 and mau-2 leads to the abnormal dorsal projection of the AVM axon, a phenotype that is novel and specific to the interaction of these two genes, indicating that mau-2 participates in the guidance of AVM by a slt-1-independent mechanism. Taken together, mau-2 defines a novel guidance factor that might be involved in the intracellular processing of guidance cues encountered by migrating cells and axons; during development.