Quercetin protects spermatogonial cells from 2,4-D-induced oxidative damage in embryonic chickens
Quercetin protects spermatogonial cells from 2,4-D-induced oxidative damage in embryonic chickens
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DOI：
10.1262/jrd.19001
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发表时间：
2007-08-01
影响因子：
1.8
通讯作者：
Taya, Kazuyoshi
中科院分区：
文献类型：
--
作者：
Mi, Yuling;Zhang, Caiqiao;Taya, Kazuyoshi
槲皮素是一种抗氧化剂黄酮类化合物，被认为对人类和动物健康有益。本研究通过分析次黄嘌呤-黄嘌呤氧化酶（HX-XO）或2,4-二氯苯氧乙酸（2,4-D）处理胚胎鸡后细胞内抗氧化系统，研究槲皮素对睾丸细胞氧化损伤的保护作用。将来自第 18 天胚胎的睾丸细胞单独用槲皮素或与 HX-XO 或 2,4-D 组合进行攻击，培养 48 小时。结果表明，槲皮素在浓度高达1.0μg/ml时对精原细胞没有表现出有害作用。暴露于 HX-XO 或 2,4-D (50 μg/ml) 会导致细胞核浓缩和细胞质空泡化，并导致睾丸细胞活力和精原细胞数量减少。乳酸脱氢酶渗漏升高导致膜完整性受损。接触 HX-XO 或 2,4-D 还会通过硫代巴比妥酸反应物质的升高以及谷胱甘肽含量和超氧化物歧化酶活性的降低而引发脂质过氧化。然而，同时补充槲皮素可将这些参数恢复到对照组的水平。因此，HX-XO 和 2,4-D 诱导精原细胞氧化应激；然而，饮食中的槲皮素可以减轻环境毒物的负面影响并恢复睾丸细胞的抗氧化系统。
Quercetin, an antioxidant flavonoid, is considered beneficial to human and animal health. In this study, the protective effects of quercetin in relation to oxidative damage of testicular cells were studied by analysis of the intracellular antioxidant system after treatment of embryonic chickens with hypoxanthine-xanthine oxidase (HX-XO) or 2,4-dichlorophenoxyacetic acid (2,4-D). Testicular cells from Day 18 embryos were challenged with quercetin alone or in combinations with HX-XO or 2,4-D for 48 h in culture. The results showed that quercetin manifested no deleterious effects on spermatogonial cells at concentrations up to 1.0 mu g/ml. Exposure to HX-XO or 2,4-D (50 mu g/ml) induced condensed nuclei and vacuolated cytoplasm and a decrease in testicular cell viability and spermatogonial cell number. Membrane integrity was damaged by elevated lactate dehydrogenase leakage. Exposure to HX-XO or 2,4-D also elicited lipid peroxidation by elevation of thiobarbituric acid reactive substances and decreased glutathione content and superoxide dismutase activity. However, simultaneous supplementation with quercetin restored these parameters to the levels in the controls. Consequently, HX-XO and 2,4-D induced oxidative stress in spermatogonial cells; however, dietary quercetin may attenuate the negative effects of environmental toxicants and restore the antioxidant system in testicular cells.