A novel transcription factor Rwdd1 and its SUMOylation inhibit the expression of sqr, a key gene of mitochondrial sulfide metabolism in Urechis unicinctus.
A novel transcription factor Rwdd1 and its SUMOylation inhibit the expression of sqr, a key gene of mitochondrial sulfide metabolism in Urechis unicinctus.
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DOI：
10.1016/j.aquatox.2018.09.012
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发表时间：
2018-11
影响因子：
4.5
通讯作者：
Xueyu Li;Xiaolong Liu;Zhenkui Qin;Maokai Wei;Xitan Hou;Tingting Zhang;Zhifeng Zhang
中科院分区：
文献类型：
--
作者：
Xueyu Li;Xiaolong Liu;Zhenkui Qin;Maokai Wei;Xitan Hou;Tingting Zhang;Zhifeng Zhang
Sulfide-quinone oxidoreductase (SQR) is a key enzyme of sulfide metabolism in metazoans, and responsible for oxidizing sulfide into thiosulfate and transmitting the generated electrons to the ubiquinone. It has been revealed that thesqrmRNA level increases significantly in echiuran wormUrechis unicinctusexposed to sulfide, and HSF1, NF1 and Sp1 have been verified to participate in its transcriptional regulation. In this study, we obtained 23 potential transcription factors interacting possibly with the proximal region (−391 to +50) ofsqrpromoter, and focused on the RWD domain-containing 1 (Rwdd1), a protein with the maximum number of clones in yeast one-hybrid (Y1H) screening, to investigate its transcriptional regulation toU. unincitus sqr. The ChIP and EMSA assays identified that the Rwdd1 can bind directly to the promoter (+18/+36) ofU. unicinctus sqr. The point mutation and transient transfection experiments discovered that TACG was the key sequence of the DNA element bound by the Rwdd1. Furthermore, theU. unicinctusRwdd1 (UuRwdd1) was identified to be a transcription repressor inhibiting thesqrpromoter activity, and the SUMOylation ofUuRwdd1 at the lysine of 90thenhanced its inhibitory effect onsqrtranscription further. Western blotting found Rwdd1 responded to sulfide in hindguts fromU. unincitus, and the protein content showed a remarkable drop in hindgut nuclei in the early sulfide exposure, and then increased significantly both in the total protein and the nuclear protein extract. We suggested that the Rwdd1 is a novel transcription factor, and these data improve our understanding of thesqrtranscriptional regulation and the mitochondrial sulfide metabolism.
