Genetic conservation of hemagglutinin gene of H9 influenza virus in chicken population in Mainland China
Genetic conservation of hemagglutinin gene of H9 influenza virus in chicken population in Mainland China
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DOI：
10.1007/s11262-004-7436-x
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发表时间：
2004-12-01
期刊：
影响因子：
1.6
通讯作者：
Kida, H
中科院分区：
文献类型：
--
作者：
Liu, JH;Okazaki, K;Kida, H
对1995年至2002年从中国内地鸡群中分离的12株H9N2型流感病毒的血凝素基因进行了分析。所有分离株在HA裂解位点都具有相同的氨基酸基序-R-S-S-R/G-L。除与其他禽流感病毒相同的保守氨基酸位于受体结合位点外，所有12株病毒的氨基酸序列均在183位有N，190位有A、T或V，137位有K，而其他H9N2型病毒的代表性毒株(DK/HK/Y280/97-like)的代表株在这些位置分别有H、E和R。这些可作为内地中国分离的H9病毒的部分分子标记。系统进化分析表明，这些分离株的HA基因属于A/DAD/Hong Kong/Y280/97类病毒谱系。自1992年内地中国暴发H9N2型流感以来，未在鸡群中发现A/QUAIL/香港/Gl/97类病毒。现有证据表明，过去几年在内地流行的H9流感病毒中国的HA基因被很好地保守。
The hemagglutinin (HA) genes of 12 H9N2 influenza virus strains isolated from chickens in Mainland China during the period 1995 - 2002 were genetically analyzed. All the isolates possessed the same amino acid motif - R-S-S-R/G-L- at the cleavage site of HA. Except for the conserved amino acids, as is the case in the other avian influenza viruses, located in the receptor binding site, all of the 12 isolates possessed N at amino acid position 183; A, T, or V at position 190; K at position 137, whereas the representative strains of the other lineage ( except Dk/HK/Y280/97-like lineage) virus of H9N2 viruses had H, E, and R at these positions respectively. These could be considered as the partial molecular markers of the H9 viruses isolated from chickens in Mainland China. Phylogenetic analyses showed HA genes of these isolates belonged to that of A/duck/Hong Kong/Y280/97-like virus lineage. No A/quail/Hong Kong/Gl/97-like virus was found in chicken, population since the outbreak of H9N2 influenza in Mainland China in 1992. The available evidence indicates that HA genes of H9 influenza virus circulating in Mainland China during the past years were well conserved.