Lactic acid production from waste sugarcane bagasse derived cellulose
Lactic acid production from waste sugarcane bagasse derived cellulose
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DOI：
10.1039/b605839f
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发表时间：
2007-01-01
期刊：
影响因子：
9.8
通讯作者：
Gokhale, Digambar V.
中科院分区：
文献类型：
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作者：
Adsul, Mukund G.;Varma, Anjani J.;Gokhale, Digambar V.
研究了利用印度丰富的生物质原料之一甘蔗渣纤维素生产L（+）乳酸。对甘蔗渣进行化学处理得到纯化的甘蔗渣纤维素样品，该样品比甘蔗渣本身更容易受到纤维素酶的攻击。该样品，在高浓度（10%）下，由纤维素酶制剂（10 FPU g（-1）纤维素）水解，这些纤维素酶制剂来自我们自己实验室产生的突变体。我们得到了最大的水解率（72%），主要的最终产物是葡萄糖和纤维素二糖。该甘蔗渣纤维素样品在含有由紫青霉突变体EU1衍生的纤维素酶制剂和由德布鲁克氏乳杆菌突变体Uc-3衍生的纤维素二糖的培养基中同时糖化和发酵（SSF）生产乳酸。以浓度为80 g l（-1）的甘蔗渣纤维素为原料，最大乳酸浓度为67 g l(-1)，最高产率和产量分别为0.93 g l(-1) h和0.83 g g（-1）。发现突变体Uc-3利用高浓度的纤维素二糖（50 g l(-1)），并以同质发酵的方式将其转化为乳酸。考虑到甘蔗渣是一种丰富的废物，我们建议将这种生物质增值以生产纤维素，然后生产糖，这些糖可以发酵成乙醇和乳酸等产品。
Production of L(+) lactic acid from sugarcane bagasse cellulose, one of the abundant biomass materials available in India, was studied. The bagasse was chemically treated to obtain a purified bagasse cellulose sample, which is much more amenable to cellulase enzyme attack than bagasse itself. This sample, at high concentration (10%), was hydrolyzed by cellulase enzyme preparations (10 FPU g(-1) cellulose) derived from mutants generated in our own laboratory. We obtained maximum hydrolysis (72%), yielding glucose and cellobiose as the main end products. Lactic acid was produced from this bagasse cellulose sample by simultaneous saccharification and fermentation (SSF) in a media containing a cellulase enzyme preparation derived from Penicillium janthinellum mutant EU1 and cellobiose utilizing Lactobacillus delbrueckii mutant Uc-3. A maximum lactic acid concentration of 67 g l(-1) was produced from a concentration of 80 g l(-1) of bagasse cellulose, the highest productivity and yield being 0.93 g l(-1) h(-1) and 0.83 g g(-1), respectively. The mutant Uc-3 was found to utilize high concentrations of cellobiose (50 g l(-1)) and convert it into lactic acid in a homo-fermentative way. Considering that bagasse is a waste material available in abundance, we propose to valorize this biomass to produce cellulose and then sugars, which can be fermented to products such as ethanol and lactic acid.