PHOTOREACTIVATING ENZYME FROM STREPTOMYCES-GRISEUS .4. ON THE NATURE OF THE CHROMOPHORIC COFACTOR IN STREPTOMYCES-GRISEUS PHOTOREACTIVATING ENZYME
PHOTOREACTIVATING ENZYME FROM STREPTOMYCES-GRISEUS .4. ON THE NATURE OF THE CHROMOPHORIC COFACTOR IN STREPTOMYCES-GRISEUS PHOTOREACTIVATING ENZYME
复制标题
DOI：
10.1111/j.1751-1097.1981.tb04298.x
复制
发表时间：
1981-01-01
影响因子：
3.3
通讯作者：
BERENDS, W
中科院分区：
文献类型：
--
作者：
EKER, APM;DEKKER, RH;BERENDS, W
根据辅助因子和参比化合物在不同ph值下的吸收光谱和荧光光谱，提出了金黄色葡萄球菌光活化酶内在辅助因子显色部分的结构。该辅助因子的主要显色部分为7,8-二甲基-8-羟基-5-去氮黄素衍生物（Ia）。从整个稻谷菌细胞中分离到一个化合物SF420 (Ic)，该化合物含有相同的显色基团。这种化合物在蓝光照射下使胸腺嘧啶二聚体的分裂增敏，支持光再激活酶辅助因子的结构。
A structure for the chromophoric part of the intrinsic cofactor of S. griseus photoreactivating enzyme is suggested on the basis of absorption and fluorescence spectra of cofactor and reference compounds at various pH. The essential chromogenic part of this cofactor is proposed to be a 7,8-didemethyl-8-hydroxy-5-deazaflavin derivative (Ia). From whole S. griseus cells a compound SF420 (Ic) was isolated which contains the same chromophoric group. This compound sensitizes the splitting of thymine dimers when irradiated with blue light, supporting the structure proposed for the photoreactivating enzyme cofactor.