Designing plasmas for chronic wound disinfection
Designing plasmas for chronic wound disinfection
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DOI：
10.1088/1367-2630/11/11/115013
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发表时间：
2009-11-26
影响因子：
3.3
通讯作者：
Morfill, G. E.
中科院分区：
文献类型：
--
作者：
Nosenko, T.;Shimizu, T.;Morfill, G. E.
低温常压等离子体辐照为慢性创面消毒提供了一种很有前途的方法。为了达到这一目的，血浆应该满足以下标准：它应该显著降低创伤区域的细菌密度，导致辐射后细菌生长的长期抑制，同时又不会对人体细胞造成任何负面影响。为了设计出能够满足这些要求的等离子体，我们评估了不同组分在Ar等离子体辐照下对杀菌性能的相对贡献。我们证明了等离子体产生的紫外线辐射是Ar等离子体短期灭菌的主要因素。另一方面，等离子体产生的活性氮物种(RNS)和活性氧物种(ROS)会在照射后对细菌生长产生长期的抑制作用，因此，对于防止两种治疗方法之间的伤口细菌再繁殖具有重要意义。我们还证明，在特定浓度下，血浆产生的RNS和ROS可显著降低细菌密度，但对人类皮肤细胞没有不良影响。讨论了等离子体产生的活性物质对细菌和人类细胞产生不同影响的可能机制。这项研究的结果表明，治疗用的Ar等离子体应该朝着降低等离子体产生的紫外线辐射强度和增加非UV等离子体产品密度的方向进行优化。
Irradiation with low-temperature atmospheric-pressure plasma provides a promising method for chronic wound disinfection. To be efficient for this purpose, plasma should meet the following criteria: it should significantly reduce bacterial density in the wounded area, cause a long-term post-irradiation inhibition of bacterial growth, yet without causing any negative effect on human cells. In order to design plasmas that would satisfy these requirements, we assessed the relative contribution of different components with respect to bactericidal properties due to irradiation with argon plasma. We demonstrate that plasma-generated UV radiation is the main short-term sterilizing factor of argon plasma. On the other hand, plasma-generated reactive nitrogen species (RNS) and reactive oxygen species (ROS) cause a long-term 'after-irradiation' inhibition of bacterial growth and, therefore, are important for preventing wound recolonization with bacteria between two treatments. We also demonstrate that at certain concentrations plasma-generated RNS and ROS cause significant reduction of bacterial density, but have no adverse effect on human skin cells. Possible mechanisms of the different effects of plasma-generated reactive species on bacteria and human cells are discussed. The results of this study suggest that argon plasma for therapeutic purposes should be optimized in the direction of reducing the intensity of plasma-generated UV radiation and increasing the density of non-UV plasma products.