Suppression of cell-spreading and phagocytic activity on nano-pillared surface: in vitro experiment using hemocytes of the colonial ascidian Botryllus schlosseri
Suppression of cell-spreading and phagocytic activity on nano-pillared surface: in vitro experiment using hemocytes of the colonial ascidian Botryllus schlosseri
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纳米柱表面细胞扩散和吞噬活性的抑制：使用群体海鞘 Botryllus schlosseri 血细胞的体外实验
DOI：
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发表时间：
2015
影响因子：
0.7
通讯作者：
E. Hirose
中科院分区：
文献类型：
--
作者：
L. Ballarin;N. Franchi;F. Gasparini;F. Caicci;A. Miyauchi;E. Hirose
纳米尺度的乳头阵列的身体表面上已经描述了从各种无脊椎动物，包括内寄生和中寄生桡足类，但乳头阵列的功能还没有得到很好的理解。以聚苯乙烯制成的纳米柱片作为模拟寄生虫体表乳头阵列的材料，研究了集群海鞘血细胞的细胞铺展和吞噬功能。在柱化表面上，每单位面积的扩散变形细胞的数量和吞噬血细胞的数量总是小于平坦表面上的那些（Mann-Whitney检验，p < 0.05 - 0.001），这可能是因为柱化表面上的细胞附着的有效面积比平坦片上的面积小得多。本研究结果支持了寄生虫体表的乳头阵列减少宿主血细胞的先天免疫反应的想法。
Nano-scale nipple array on the body surface has been described from various invertebrates including endoparasitic and mesoparasitic copepods, but the functions of the nipple array is not well understood. Using the hydrophilized nanopillar sheets made of polystyrene as a mimetic material of the nipple arrays on the parasites’ body surface, we assayed the cell spreading and phagocytosis of the hemocytes of the colonial ascidian Botryllus schlosseri. On the pillared surface, the number of spreading amebocytes and the number of phagocytizing hemocytes per unit area were always smaller than those on the flat surface (Mann-Whitney test, p < 0.05 - 0.001), probably because the effective area for the cell attachment on the pillared surface is much smaller than the area on the flat sheet. The present results supports the idea that the nipple array on the parasites' body surface reduces the innate immune reaction from the host hemocytes.
DOI：
10.1098/rspb.2005.3369
发表时间：
2006-03-22
影响因子：
4.7
作者：
Stavenga, DG;Foletti, S;Arikawa, K
通讯作者：
Arikawa, K
外衣乳头阵列是否可以用来伪装白天的樽海鞘？
影响因子：
1.2
作者：
E. Hirose;D. Sakai;T. Shibata;J. Nishii;H. Mayama;A. Miyauchi;J. Nishikawa
通讯作者：
J. Nishikawa