Honokiol suppresses mycelial growth and reduces virulence of Botrytis cinerea by inducing autophagic activities and apoptosis
Honokiol suppresses mycelial growth and reduces virulence of Botrytis cinerea by inducing autophagic activities and apoptosis
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DOI：
10.1016/j.fm.2019.103411
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发表时间：
2020-06-01
影响因子：
5.3
通讯作者：
Chen, Tong
中科院分区：
文献类型：
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作者：
Ma, Danying;Cui, Xiaomin;Chen, Tong
真菌病原菌导致严重的品质恶化和产量损失，迫切需要探索有效的措施来控制植物采前和采后阶段的真菌病害。因此，针对替代抗菌靶点的天然物质的研究已成为研究热点。在这里，我们发现和厚朴酚（一种从厚朴中提取的多酚化合物）通过诱导自噬活性和细胞凋亡，显着抑制菌丝生长并降低灰霉病菌对收获果实的毒力。此外，和厚朴酚能够消除线粒体膜电位并诱导活性氧的积累。一些与水果致病性相关的关键基因也被发现显着下调。总之，和厚朴酚作为一种针对自噬和凋亡机制的替代药物来控制灰霉病的发生是有效的，这可能会进一步丰富作物管理者对抗由这种真菌和类似真菌引起的采后疾病的工具包。
Fungal pathogens lead to severe quality deterioration and yield loss, making it urgent to explore efficient measures to control fungal diseases at the preharvest and postharvest stages of plants. Therefore, studies on natural substances targeting alternative antimicrobial targets have become hot spots of research. Here, we show that honokiol, a polyphenolic compound obtained from Magnolia officinalis, significantly suppressed mycelial growth and reduced virulence of B. cinerea on harvested fruit by inducing autophagic activities and apoptosis. Moreover, honokiol was capable of abolishing the mitochondrial membrane potential and inducing the accumulation of reactive oxygen species. Some key genes involved in pathogenicity on fruit were also found significantly down-regulated. In summary, honokiol was effective as an alternative agent targeting autophagic and apoptotic machineries to control the incidence of gray mold, which may further enrich the toolkit of crop managers for fighting postharvest diseases caused by this and similar fungi.