Primitive roles for inhibitory interneurons in developing frog spinal cord
Primitive roles for inhibitory interneurons in developing frog spinal cord
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DOI：
10.1523/jneurosci.1633-04.2004
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发表时间：
2004-06-23
影响因子：
5.3
通讯作者：
Soffe, SR
中科院分区：
文献类型：
--
作者：
Li, WC;Higashijima, S;Soffe, SR
哺乳动物脊髓神经元网络的多样性及其连接阻碍了对其神经网络的理解。低级脊椎动物发育过程中更简单的网络可能会提供对基本组织的见解。为了研究爪蟾蝌蚪脊髓抑制性中间神经元的功能，采用成对全细胞记录的方法。我们直接展示了一类中间神经元，具有独特的解剖结构，产生甘氨酸能，负反馈抑制，可以限制运动神经元和中央模式发生器的中间神经元在游泳过程中的放电。这些相同的神经元也在游泳时产生感觉通路的抑制性门控。这一发现提出了一种可能性，即在发育后期具有不同功能的中间神经元可能与早期共享这些功能的中间神经元有所区别。初步证据表明，这些抑制性中间神经元表达了转录因子engrailed，这支持了发育中的斑马鱼中间神经元可能具有同源性，这些中间神经元也表达engrailed，并且具有非常相似的解剖结构和功能。
Understanding the neuronal networks in the mammal spinal cord is hampered by the diversity of neurons and their connections. The simpler networks in developing lower vertebrates may offer insights into basic organization. To investigate the function of spinal inhibitory interneurons in Xenopus tadpoles, paired whole-cell recordings were used. We show directly that one class of interneuron, with distinctive anatomy, produces glycinergic, negative feedback inhibition that can limit firing in motoneurons and interneurons of the central pattern generator during swimming. These same neurons also produce inhibitory gating of sensory pathways during swimming. This discovery raises the possibility that some classes of interneuron, with distinct functions later in development, may differentiate from an earlier class in which these functions are shared. Preliminary evidence suggests that these inhibitory interneurons express the transcription factor engrailed, supporting a probable homology with interneurons in developing zebrafish that also express engrailed and have very similar anatomy and functions.