L-cysteine functionalized straticulate C3N4 for the selective enrichment of glycopeptides
L-cysteine functionalized straticulate C3N4 for the selective enrichment of glycopeptides
复制标题
L-半胱氨酸功能化层状 C3N4 用于选择性富集糖肽
DOI：
10.1016/j.chroma.2019.460545
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发表时间：
2020-01-11
影响因子：
4.1
通讯作者：
Ying, Wantao
中科院分区：
文献类型：
--
作者：
Zhang, Yong;Jing, Hongyu;Ying, Wantao
The facile enrichment of glycopeptides or glycoproteins poses great challenges for glycoproteomic research. In this study, a novel hydrophilic material, named zwitterionic hydrophilic L-cysteine derivatized straticulate-C3N4 composites (LCAC), were synthesized and evaluated for the enrichment of N-glycopeptides. LCAC exhibited good biocompatibility, excellent hydrophilicity and selectivity, by virtue of the large surface of C3N4 and the zwitterionic property offered by cysteine. LCAC demonstrated excellent performance for N-glycopeptide enrichment with the sensitivity of 0.033 fmol/mu L, selectivity of 1:100, and high recovery rate (similar to 85%). The performance of LCAC was demonstrated by the identification of 35 N-glycopeptides from IgG, as well as capturing 1809 human urine N-glycopeptides corresponding to 876 N-glycoproteins. Comparing the LCAC with our developed phenylboronic acid functionalized material showed a certain complementary due to the different binding mechanism. The simple production and enhanced hydrophilic properties make the material a promising choice for glycoproteomics researches. (C) 2019 Elsevier B.V. All rights reserved.
